Suppressive effects of Bacillus spp. on mycelia, apothecia and sclerotia formation of Sclerotinia sclerotiorum and potential as biological control of white mold on mustard

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• 作者：Md Muzahid E Rahman ; Delwar M. Hossain ; Kazuki Suzuki…
• 关键词：Bacillus amyloliquefaciens ; Biocontrol ; Plant growth promoting activity ; Sclerotinia sclerotiorum
• 刊名：Australasian Plant Pathology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：45
• 期：1
• 页码：103-117
• 全文大小：4,976 KB
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• 作者单位：Md Muzahid E Rahman (1) (2) (3)
  Delwar M. Hossain (2)
  Kazuki Suzuki (3)
  Ayaka Shiiya (3)
  Kazushi Suzuki (4)
  Tapan Kumar Dey (1)
  Masanori Nonaka (4)
  Naoki Harada (4)
  
  1. Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur, 1701, Bangladesh
  2. Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh
  3. Graduate School of Science and Technology, Niigata University, Niigata, 950-2181, Japan
  4. Institute of Science and Technology, Niigata University, Niigata, 950-2181, Japan
  
• 刊物主题：Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1448-6032

文摘

Biological control, especially with Bacillus-based biocontrol agents, offers an attractive alternative to synthetic pesticides for sustainable management of white mold disease caused by Sclerotinia sclerotiorum. In this study, eight effective Bacillus isolates were isolated from rhizospheric soil samples as potential bacterial biocontrol agents. Cultural, biochemical, and molecular analyses of 16S rDNA and gyrase subunit A (gyrA) confirmed that all isolates were identified as Bacillus amyloliquefaciens subsp. plantarum. The production of hydrolytic enzymes and the plant growth-promotional attributes of these isolates confirmed their multifaceted potential. Molecular analysis of the eight biosynthetic genes, which are related to antibiotic properties of bacilli, revealed that all of the isolates possess five genes: bacA for bacilysin, dfnM for difficidin, fenA for fengycin, ituA for iturin, and sfp for surfactin. The Bacillus isolates inhibited mycelial growth and suppressed formation of sclerotia during an in vitro test against S. sclerotiorum. Deformities and cell-wall lysis of mycelia, abnormalities of apothecia, and germination failure of ascospores through interaction with the Bacillus isolates were observed with light and scanning electron microscopes, suggesting that they have high antagonistic effects against S. sclerotiorum. Seed bacterization with the Bacillus isolates protected mustard seedlings in vitro up to 98 % against S. sclerotiorum. In a pot experiment, damages of mustard plants against the pathogen decreased up to 90 % after foliar spray of the Bacillus isolates. In addition, the isolates increased seed germination and accelerated seedling vigor of mustard, suggesting that they have plant growth-promoting functions. Keywords Bacillus amyloliquefaciens Biocontrol Plant growth promoting activity Sclerotinia sclerotiorum