Establishment of an immortal cynomolgus macaque fibroblast cell line for propagation of cynomolgus macaque cytomegalovirus (CyCMV)
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- 作者：Aruna P. Ambagala (1) (6)
Angie K. Marsh (1) (3)
Jacqueline K. Chan (1)
Rosemarie Mason (1)
Richard Pilon (4)
Jocelyn Fournier (5)
Paul Sandstrom (4)
David O. Willer (1)
Kelly S. MacDonald (1) (3) - 刊名：Archives of Virology
- 出版年：2013
- 出版时间：May 2013
- 年：2013
- 卷：158
- 期：5
- 页码：955-965
- 全文大小：646KB
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Angie K. Marsh (1) (3)
Jacqueline K. Chan (1)
Rosemarie Mason (1)
Richard Pilon (4)
Jocelyn Fournier (5)
Paul Sandstrom (4)
David O. Willer (1)
Kelly S. MacDonald (1) (3)
1. Department of Microbiology, Mount Sinai Hospital, Toronto, ON, M5G 1X5, Canada
6. Canadian Food Inspection Agency-Lethbridge Laboratory, National Centres for Animal Disease, TWP Rd 9-1, Lethbridge, AB, T1J 3Z4, Canada
3. Department of Medicine, University of Toronto, Toronto, ON, M5S 1A8, Canada
4. National HIV and Retrovirology Laboratories, National Microbiology Laboratory, Public Health Agency of Canada, Ottawa, ON, K1A 0L2, Canada
5. Scientific Services Division, Health Products and Food Branch, Health Canada, Ottawa, ON, K1A 0L2, Canada - ISSN：1432-8798
文摘
Cynomolgus macaques are widely used as an animal model in biomedical research. We have established an immortalized cynomolgus macaque fibroblast cell line (MSF-T) by transducing primary dermal fibroblasts isolated from a 13-year-old male cynomolgus macaque with a retrovirus vector expressing human telomerase reverse transcriptase (hTERT). The MSF-T cells showed increased telomerase enzyme activity and reached over 200 in vitro passages compared to the non-transduced dermal fibroblasts, which reached senescence after 43 passages. The MSF-T cell line is free of mycoplasma contamination and is permissive to the newly identified cynomolgus macaque cytomegalovirus (CyCMV). CyCMV productively infects MSF-T cells and induces down-regulation of MHC class I expression. The MSF-T cell line will be extremely useful for the propagation of CyCMV and other cynomolgus herspesviruses in host-derived fibroblast cells, allowing for the retention of host-specific viral genes. Moreover, this cell line will be beneficial for many in vitro experiments related to this animal model.