Patterns of cancer cell sphere formation in primary cultures of human oral tongue squamous cell carcinoma and neck nodes

详细信息    查看全文

• 作者：Saira Saleem (1)
  Arif Jamshed (2)
  Saima Faisal (3)
  Raza Hussain (4)
  Muhammad Tahseen (5)
  Asif Loya (5)
  Chris Sutton (6)
  
  1. Basic Sciences Research Department ; Shaukat Khanum Memorial Cancer Hospital and Research Center ; 7-A Block R-3 ; Johar Town ; Lahore ; 54000 ; Pakistan
  2. Department of Radiation Oncology ; Shaukat Khanum Memorial Cancer Hospital and Research Center ; 7-A Block R-3 ; Johar Town ; Lahore ; 54000 ; Pakistan
  3. Clinical Research Office ; Shaukat Khanum Memorial Cancer Hospital and Research Center ; 7-A Block R-3 ; Johar Town ; Lahore ; 54000 ; Pakistan
  4. Department of Surgical Oncology ; Shaukat Khanum Memorial Cancer Hospital and Research Center ; 7-A Block R-3 ; Johar Town ; Lahore ; 54000 ; Pakistan
  5. Department of Pathology ; Shaukat Khanum Memorial Cancer Hospital and Research Center ; 7-A Block R-3 ; Johar Town ; Lahore ; 54000 ; Pakistan
  6. Institute of Cancer Therapeutics ; University of Bradford ; Tumbling hill street ; Bradford ; BD7 1BD ; United Kingdom
  
• 关键词：Oral tongue squamous cell carcinoma ; Lymph node metastasis ; Primary culture ; Cancer cell sphere ; Cancer stem cells ; in vitro assay
• 刊名：Cancer Cell International
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：1,546 KB
• 参考文献：1. Noronha, V, Tsomo, U, Jamshed, A, Hai, M, Wattegama, S, Baral, R, Piya, M, Prabhash, K (2012) A fresh look at oncology facts on south central Asia and SAARC countries. South Asian J Cancer 1: pp. 1-4 CrossRef
  2. Jamshed, A, Syed, AA, Shah, MA, Jamshed, S (2013) Improving cancer care in Pakistan. South Asian J Cancer 2: pp. 36-37 CrossRef
  3. Akram, S, Mirza, T, Aamir Mirza, M, Qureshi, M (2013) Emerging Patterns in Clinico-pathological spectrum of Oral Cancers. Pak J Med Sci 29: pp. 783-787
  4. Clarke, M, Dick, J, Dirks, P, Eaves, C, Jamieson, C, Jones, D, Visvader, J, Weissman, I,, Wahl, G (2006) Cancer stem cells鈥損erspectives on current status and future directions: AACR Workshop on cancer stem cells. Cancer Res 66: pp. 9339-9344 CrossRef
  5. Bonnet, D, Dick, J (1997) Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nat Med 3: pp. 730-737 CrossRef
  6. Al-Hajj, M, Wicha, M, Benito-Hernandez, A, Morrison, S, Clarke, M (2003) Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci U S A 100: pp. 3983-3988 CrossRef
  7. Ponti, D, Costa, A, Zaffaroni, N, Pratesi, G, Petrangolini, G, Coradini, D, Pilotti, S, Pierotti, MA, Daidone, MG (2005) Isolation and In vitro Propagation of Tumorigenic Breast Cancer Cells with Stem/Progenitor Cell Properties. Cancer Res 65: pp. 5506-5511 CrossRef
  8. Singh, SK, Clarke, ID, Terasaki, M, Bonn, VE, Hawkins, C, Squire, J, Dirks, PB (2003) Identification of a Cancer Stem Cell in Human Brain Tumors. Cancer Res 63: pp. 5821-5828
  9. Patrawala, L, Calhoun, T, Schneider-Broussard, R, Li, H, Bhatia, B, Tang, S, Reilly, JG, Chandra, D, Zhou, J, Claypool, K (2006) Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells. Oncogene 25: pp. 1696-1708 CrossRef
  10. Li, C, Heidt, DG, Dalerba, P, Burant, CF, Zhang, L, Adsay, V, Wicha, M, Clarke, MF, Simeone, DM (2007) Identification of Pancreatic Cancer Stem Cells. Cancer Res 67: pp. 1030-1037 CrossRef
  11. Prince, ME, Sivanandan, R, Kaczorowski, A, Wolf, GT, Kaplan, MJ, Dalerba, P, Weissman, IL, Clarke, MF, Ailles, LE (2007) Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci 104: pp. 973-978 CrossRef
  12. Costea, DE, Tsinkalovsky, O, Vintermyr, OK, Johannessen, AC, Mackenzie, IC (2006) Cancer stem cells - new and potentially important targets for the therapy of oral squamous cell carcinoma. Oral Dis 12: pp. 443-454 CrossRef
  13. Harper, LJ, Piper, K, Common, J, Fortune, F, Mackenzie, IC (2007) Stem cell patterns in cell lines derived from head and neck squamous cell carcinoma. J Oral Pathol Med 36: pp. 594-603 CrossRef
  14. Luo, X, Okubo, T, Randell, S, Hogan, BL (2009) Culture of endodermal stem/progenitor cells of the mouse tongue. In Vitro Cell Dev Biol Anim 45: pp. 44-54 CrossRef
  15. Locke, M, Heywood, M, Fawell, S, Mackenzie, IC (2005) Retention of Intrinsic Stem Cell Hierarchies in Carcinoma-Derived Cell Lines. Cancer Res 65: pp. 8944-8950 CrossRef
  16. Ponta, H, Sherman, L, Herrlich, PA (2003) CD44: From adhesion molecules to signalling regulators. Nat Rev Mol Cell Biol 4: pp. 33-45 CrossRef
  17. Joshua, B, Kaplan, MJ, Doweck, I, Pai, R, Weissman, IL, Prince, ME, Ailles, LE (2012) Frequency of cells expressing CD44, a Head and Neck cancer stem cell marker: Correlation with tumor aggressiveness. Head Neck 34: pp. 42-49 CrossRef
  18. Jaggupilli, A (2012) Significance of CD44 and CD24 as Cancer Stem Cell Markers: An Enduring Ambiguity. Clin Dev Immunol 2012: pp. 11 CrossRef
  19. Baumann, P, Cremers, N, Kroese, F, Orend, G, Chiquet-Ehrismann, R, Uede, T, Yagita, H, Sleeman, JP (2005) CD24 Expression Causes the Acquisition of Multiple Cellular Properties Associated with Tumor Growth and Metastasis. Cancer Res 65: pp. 10783-10793 CrossRef
  20. Prince, MEP, Ailles, LE (2008) Cancer Stem Cells in Head and Neck Squamous Cell Cancer. J Clin Oncol 26: pp. 2871-2875 CrossRef
  21. Leemans, CR, Tiwari, R, Nauta, JJ, Waal, I, Snow, GB (1993) Regional lymph node involvement and its significance in the development of distant metastases in head and neck carcinoma. Cancer 71: pp. 452-456 CrossRef
  22. Wyckoff, J, Wang, W, Lin, EY, Wang, Y, Pixley, F, Stanley, ER, Graf, T, Pollard, JW, Segall, J, Condeelis, J (2004) A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. Cancer Res 64: pp. 7022-7029 CrossRef
  23. LJ, van 't Veer, Dai, H, Vijver, MJ, He, YD, Hart, AA, Mao, M, Peterse, HL, van der Kooy, K, Marton, MJ, Witteveen, AT (2002) Gene expression profiling predicts clinical outcome of breast cancer. Nature 415: pp. 530-536 CrossRef
  24. Yang, J, Mani, SA, Donaher, JL, Ramaswamy, S, Itzykson, RA, Come, C, Savagner, P, Gitelman, I, Richardson, A, Weinberg, RA (2004) Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Cell 117: pp. 927-939 CrossRef
  
• 刊物主题：Cancer Research; Cell Biology;
• 出版者：BioMed Central
• ISSN：1475-2867

文摘

Recently a sub-population of cells with stem cell characteristics, reported to be associated with initiation, growth, spread and recurrence, has been identified in several solid tumors including oral tongue squamous cell carcinoma (OTSCC). The aim of our pilot study was to isolate CD44+ cancer stem cells from primary cultures of OTSCC and neck node Level I (node-I) biopsies, grow cell spheres and observe their characteristics in primary cultures. Parallel cultures of hyperplastic lesions of tongue (non-cancer) were set up as a control. Immunohistochemistry was used to detect CD44/CD24 expression and magnetic activated cell sorting to isolate CD44+ cell populations followed by primary cell culturing. Both OTSCC and node-I biopsies produced floating spheres in suspension, however those grown in hyperplastic and node-I primary cultures did not exhibit self-renewal properties. Lymph node metastatic OTSCC, express higher CD44/CD24 levels, produce cancer cell spheres in larger number and rapidly (24 hours) compared to node negative OTSCC (1 week) and non-cancer specimens (3 weeks). In addition, metastatic OTSCC have the capacity for proliferation for up to three generations in primary culture. This in vitro system will be used to study cancer stem cell behavior, therapeutic drug screening and optimization of radiation dose for elimination of resistant cancer cells.