Transgenic Turfgrasses Expressing Hyperactive Ser599Ala Phytochrome A Mutant Exhibit Abiotic Stress Tolerance

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• 作者：Mayank Anand Gururani ; Markkandan Ganesan ; In-Ja Song…
• 关键词：Abiotic stress ; Heavy metal ; Phytochrome ; Salinity ; Turfgrass
• 刊名：Journal of Plant Growth Regulation
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：35
• 期：1
• 页码：11-21
• 全文大小：1,517 KB
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• 作者单位：Mayank Anand Gururani (1) (4)
  Markkandan Ganesan (1) (2)
  In-Ja Song (1)
  Yunjeong Han (3)
  Jeong-Il Kim (3)
  Hyo-Yeon Lee (1)
  Pill-Soon Song (1)
  
  1. Faculty of Biotechnology, Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756, Korea
  4. School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Korea
  2. Department of Biological Sciences, Presidency University, Kolkata, 700073, West Bengal, India
  3. Department of Biotechnology and Kumho Life Science Laboratory, Chonnam National University, Gwangju, 500-757, Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Cell Biology
  Agriculture
  Forestry
  
• 出版者：Springer New York
• ISSN：1435-8107

文摘

Turfgrasses are environmentally and recreationally valuable plants that are constantly subjected to various forms of stress in their artificial and natural habitats. Previously, it was shown that the transformation of a hyperactive mutant (Serine 599 Alanine, S599A) of oat phytochrome A in zoysia grass (Zoysia japonica) and creeping bentgrass (Agrostis stolonifera L.) resulted in superior quality turfgrass with improved shade tolerance response. We now examined the abiotic stress response of the transgenic turfgrasses expressing the hyperactive mutant S599A-PhyA. The transgenic S599A-PhyA plants subjected to high salinity and heavy metal toxicity stress exhibited higher chlorophyll content, lower hydrogen peroxide level, and higher proline accumulation than the controls. Furthermore, the anti-oxidative activities of four reactive oxygen species scavenging enzymes and the total biomass (above and below-ground) were higher in S599A-PhyA plants than in the controls under both the stress conditions. Moreover, higher photosynthetic efficiency (F _v/F _m) of S599A-PhyA plants indicated healthier growth than the controls under stress conditions. Results suggest that the hyperactive mutant of oat phytochrome A confers abiotic stress tolerance in plants, and can be used to efficiently develop abiotic stress tolerant crops in future.