Relative expression of apocarotenoid biosynthetic genes in developing stigmas of Crocus sativus L.

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• 作者：Javid IqbaLMzr (1)
  Nazeer Ahmed (2)
  Tassem Ahmad Mokhdomi (2)
  Asrar Hussain Wafai (1)
  Sajad Hassan Wani (1)
  Shoiab Bukhari (1)
  Asif Amin (1)
  Raies Ahmad Qadri (1)
  
• 关键词：Crocus sativus ; CsBCH ; CsGT ; 2 ; CsLYC ; CsZCD ; stigma development
• 刊名：Journal of Crop Science and Biotechnology
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：16
• 期：3
• 页码：183-188
• 全文大小：
• 作者单位：Javid IqbaLMzr (1)
  Nazeer Ahmed (2)
  Tassem Ahmad Mokhdomi (2)
  Asrar Hussain Wafai (1)
  Sajad Hassan Wani (1)
  Shoiab Bukhari (1)
  Asif Amin (1)
  Raies Ahmad Qadri (1)
  
  1. University of Kashmir, Srinagar, J & K, India, 190006
  2. Central Institute of Temperate Horticulture, Srinagar, J & K, India, 190007
  
• ISSN：2005-8276

文摘

Saffron, the desiccated stigmas of Crocus sativus, is recognized for its attractive color, flavor, and aroma which are due to the accumulation of crocin, picrocrocin, and safranal, respectively. HPLC analysis demonstrated maximum apocarotenoid accumulation during the fully developed scarlet stage of stigma development followed by the orange and yellow stages of stigma development. Reverse Transcription-PCR analysis revealed a concurrent expression pattern of CsZCD and CsLYC genes in a developmental stagespecific manner. However, CsBCH and CsGT2 genes were specifically expressed during the mature, scarlet stage of stigma development. Real-Time PCR analysis showed a sharp increase in gene expression of CsLYC gene during stigma development indicative of its possible regulatory role in apocarotenoid biosynthesis or stigma development. Results suggest that genetic manipulation of this gene can help to improve the quality of stigma in saffron; besides highlighting its potential to monitor stigma development during in vitro experimentation.