Molecularly imprinted polymer beads for clean-up and preconcentration of β-lactamase-resistant penicillins in milk
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- 作者：Javier L. Urraca ; Raquel Chamorro-Mendiluce…
- 关键词：Penicillin ; Molecularly imprinted polymers ; Surrogate template ; MISPE ; Milk ; Method validation
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：408
- 期：7
- 页码：1843-1854
- 全文大小：856 KB
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Raquel Chamorro-Mendiluce (3)
Guillermo Orellana (3)
Maria C. Moreno-Bondi (1)
1. Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, 28040, Madrid, Spain
2. CEI Campus Moncloa, UCM-UPM, Avda Complutense s/n, 28040, Madrid, Spain
3. Department of Organic Chemistry, Faculty of Chemistry, Universidad Complutense de Madrid, 28040, Madrid, Spain - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Food Science
Inorganic Chemistry
Physical Chemistry
Monitoring, Environmental Analysis and Environmental Ecotoxicology - 出版者：Springer Berlin / Heidelberg
- ISSN：1618-2650
文摘
This work describes the development and application of class-selective molecularly imprinted polymers (MIPs) for the analysis of beta-lactamase-resistant penicillins, namely cloxacillin (CLOXA), oxacillin (OXA), and dicloxacillin (DICLOXA), in milk samples. Our method is based on molecularly imprinted solid-phase extraction (MISPE) coupled to high-performance liquid chromatography (HPLC) with diode-array detection (DAD). 2-Biphenylylpenicillin (2BPEN), a surrogate with a close resemblance to beta-lactamase-resistant penicillins in terms of size, shape, hydrophobicity, and functionality, was synthesized and used as the template for the polymer synthesis. A MIP library was prepared and screened to select the optimum functional monomer, N-(2-aminoethyl)methacrylamide, and cross-linker, trimethylolpropane trimethacrylate, that provided the best recognition for the target antibiotics. For the MISPE application, the MIPs were prepared in the form of microspheres, using porous silica beads (40–75 μm) as sacrificial scaffolds. The developed MISPE method enables efficient extraction from aqueous samples and analysis of the antimicrobials, when followed by a selective washing with 2 mL acetonitrile–water (20:80 v/v) and elution with 1 mL 0.05 mol L−1 tetrabutylammonium in methanol. The analytical method was validated according to EU guideline 2002/657/EC. The limits of quantification (S/N = 10) were in the 5.3–6.3 μg kg−1 range, well below the maximum residue limits (MRLs) currently established. Inter-day mean recoveries were in the range 99–102 % with RSDs below 9 %, improving on the performance of previously reported MISPE methods for the analysis of CLOXA, OXA, or DICLOXA in milk samples.