Molecularly imprinted polymers applied to the clean-up of zearalenone and ¦Á-zearalenol from cereal and swine feed sample extracts

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• 作者：Javier L. Urraca ; Mar¨ªa Dolores Marazuela and Mar¨ªa C. Moreno-Bondi
• 关键词：Zearalenone ; α ; Zearalenol ; Molecularly imprinted polymer ; MISPE ; Cereal ; Swine feed
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2006
• 出版时间：August, 2006
• 年：2006
• 卷：385
• 期：7
• 页码：1155-1161
• 全文大小：162 KB

文摘

A molecularly imprinted polymer prepared using 1-allylpiperazine (1-ALPP) as the functional monomer, trimethyltrimethacrylate (TRIM) as the crosslinker and the zearalenone (ZON)-mimicking template cyclododecanyl-2,4-dihydroxybenzoate (CDHB) has been applied to the clean-up and preconcentration of this mycotoxin (zearalenone) and a related metabolite, α-zearalenol (α-ZOL), from cereal and swine feed sample extracts. The extraction of ZON and α-ZOL from the food samples was accomplished using pressurized liquid extraction (PLE) with MeOH/ACN (50:50, v/v) as the extraction solvent, at 50 ¡ãC and 1500 psi. The extracted samples were cleaned up and preconcentrated through the MIP cartridge and analyzed using HPLC with fluorescence detection (λ _exc=271/ λ _em=452 nm). The stationary phase was a polar endcapped C₁₈ column, and ACN/MeOH/water 10/55/35 (v/v/v, 15 mM ammonium acetate) at a flow rate of 1.0 mL min−1 was used as the mobile phase. The method was applied to the analysis of ZON and α-ZOL in wheat, corn, barley, rye, rice and swine feed samples fortified with 50, 100 and 400 ng g−1 of both mycotoxins, and it gave recoveries of between 85 and 97 % (RSD 2.1–6.7 % , n=3) and 87–97 % (RSD 2.3–5.6 % , n=3) for α-ZOL and ZON, respectively. The method was validated using a corn reference material for ZON.