Static magnetic field controls cell cycle in cultured human glioblastoma cells
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- 作者：Seung Chan Kim ; Wooseok Im ; Jay Yong Shim ; Seung-Ki Kim ; Beom Jin Kim
- 关键词：Static magnetic field ; Glioblastoma ; Viability ; Cell cycle ; Specified pattern
- 刊名：Cytotechnology
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：68
- 期：6
- 页码：2745-2751
- 全文大小：1,244 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Biomedicine
Biochemistry - 出版者：Springer Netherlands
- ISSN：1573-0778
- 卷排序：68
文摘
Magnetic field has been widely used in clinical diagnostics or for clinical treatment and is an important biomedical technology. Glioblastoma multiforme U87 and U251 are models of a fast growing malignant cancer. We focused on cellular level drafting of these cell lines as a time-dependent effect indicator of static magnetic fields (2000 ± 600 Gauss) by using their fast-growing properties. Cell viability showed a significant decrease (p < 0.01). The results coincided with the occurrence of apoptotic signals or protein expression of cyclin B1 and cyclin dependent kinase 1 in a non-apoptotic manner. Cdk1 was decreased in proportion to ankyrin G and cyclin B1 (Chi-square test, p = 0.0366). Our findings suggest that static magnetic stimulation creates a specific cyto-proliferative pattern, rather than producing randomized growth impairment.