Rapid Nonsynonymous Evolution of the Iron-Sulfur Protein in Anthropoid Primates

详细信息    查看全文

• 作者：Jeffrey W. Doan ; Timothy R. Schmidt ; Derek E. Wildman ; Morris Goodman ; Mark L. Weiss and Lawrence I. Grossman
• 关键词：Complex III ; mitochondria ; co ; evolution ; molecular evolution ; subunit 9 ; ubiquinol– ; cytochrome creductase
• 刊名：Journal of Bioenergetics and Biomembranes
• 出版年：2005
• 出版时间：February 2005
• 年：2005
• 卷：37
• 期：1
• 页码：35-41
• 全文大小：173 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Bioorganic Chemistry
  Biochemistry
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Organic Chemistry
  
• 出版者：Springer New York
• ISSN：1573-6881

文摘

Cytochrome c (CYC) and 9 of the 13 subunits of cytochrome c oxidase (complex IV; COX) were previously shown to have accelerated rates of nonsynonymous substitution in anthropoid primates. Cytochrome b, the mtDNA encoded subunit of ubiquinol-cytochrome c reductase (complex III), also showed an accelerated nonsynonymous substitution rate in anthropoid primates but rate information about the nuclear encoded subunits of complex III has been lacking.We now report that phylogenetic and relative rates analysis of a nuclear encoded catalytically active subunit of complex III, the ironsulfur protein (ISP), shows an accelerated rate of amino acid replacement similar to cytochrome b. Because both ISP and subunit 9, whose function is not directly related to electron transport, are produced by cleavage into two subunits of the initial translation product of a single gene, it is probable that these two subunits of complex III have essentially identical underlying rates of mutation. Nevertheless, we find that the catalytically active ISP has an accelerated rate of amino acid replacement in anthropoid primates whereas the catalytically inactive subunit 9 does not.