Physiological characteristics and metabolomics of transgenic wheat containing the maize C₄ phosphoenolpyruvate carboxylase (PEPC) gene under high temperature stress

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• 作者：Xueli Qi ; Weigang Xu ; Jianzhou Zhang ; Rui Guo ; Mingzhong Zhao ; Lin Hu…
• 关键词：Wheat ; Heat tolerance ; Phosphoenolpyruvate carboxylase (PEPC) ; Photosynthesis ; Metabolomic
• 刊名：Protoplasma
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：254
• 期：2
• 页码：1017-1030
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Cell Biology; Plant Sciences; Zoology;
• 出版者：Springer Vienna
• ISSN：1615-6102
• 卷排序：254

文摘

In this paper, two transgenic wheat lines, PC27 and PC51, containing the maize PEPC gene and its wild-type (WT) were used as experimental material to study the effects of high temperature on their photosynthetic physiological characteristics and metabolome. The results showed that transgenic wheat lines had higher photosynthetic rate (P_n) than WT under non-stress treatment (NT) and high temperature stress treatment (HT), and more significantly under HT. The change trends of F_v/F_m, Ф_PSII, and q_P were similar to P_n, whereas that of non-photochemical quenching (NPQ) was the opposite. Compared with WT, no differences in chlorophyll content between the transgenic wheat and WT were observed under NT, but two transgenic lines had relatively higher contents than WT under HT. The change trends of Chlorophyll a/b radio, the decreased values of F_m, W_k, and V_j, and the activity of the antioxidant enzyme were consistent with the chlorophyll content. Compared with WT, transgenic wheat lines exhibited lower rate of superoxide anion production, H₂O₂ and malondialdehyde content under HT, and no significant differences were observed under NT. The expression pattern of the ZmPEPC gene and wheat endogenous photosynthesis-related genes were in agreement with that of P_n. Compared with WT, about 13 different metabolites including one organic acid, six amino acids, four sugars, and two polyols were identified under NT; 25 different metabolites including six organic acids, 12 amino acids, four sugars, and three polyols were identified under HT. Collectively, our results indicate that ZmPEPC gene can enhance photochemical and antioxidant enzyme activity, upregulate the expression of photosynthesis-related genes, delay degradation of chlorophyll, change contents of proline and other metabolites in wheat, and ultimately improves its heat tolerance.