Identification by deep sequencing and profiling of conserved and novel hickory microRNAs involved in the graft process

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• 作者：Xiaojiao Sima ; Bo Jiang ; Jia Fang ; Yongqin He…
• 关键词：MicroRNA ; Hickory ; Solexa sequencing ; Graft
• 刊名：Plant Biotechnology Reports
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：9
• 期：3
• 页码：115-124
• 全文大小：504 KB
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• 作者单位：Xiaojiao Sima (1) (2)
  Bo Jiang (1) (2)
  Jia Fang (1) (2)
  Yongqin He (1) (2)
  Zhongxiang Fang (1) (2)
  Saravana Kumar KM (1) (2)
  Wei Ren (1) (2)
  Lingling Qiu (1) (2)
  Xiaomin Chen (1) (2)
  Bingsong Zheng (1) (2)
  
  1. Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People’s Republic of China
  2. Center for Cultivation of Subtropical Forest Resources (CCSFR), Zhejiang A & F University, Dong Hu Campus, 88 Northern Circle Road, Linan, Hangzhou, 311300, People’s Republic of China
  
• 刊物主题：Plant Sciences; Cell Biology; Plant Biochemistry; Biotechnology; Agriculture;
• 出版者：Springer Japan
• ISSN：1863-5474

文摘

MicroRNAs (miRNAs) play a vital role in plant development and growth through negative regulation of post-transcriptional gene expression. Carya cathayensis (hickory) is an important species for dried nuts and oil in China, with high nutritional and economic value. The graft technique is an important strategy for hickory cultivation. To understand the role of miRNAs involved in the hickory graft process, we constructed three small ribonucleic acid (RNA) libraries from hickory rootstock (2?years old) and scion (1?year old) at 0, 7, and 14?days post-graft. Sequence analysis of the three libraries identified 21 conserved miRNAs belonging to 13 families, and 10 novel and 8 potentially novel miRNAs belonging to 15 families. Among these miRNAs, 12 miRNAs were differentially expressed during the graft process in hickory and two-thirds were downregulated. Quantitative real-time polymerase chain reaction validated that 14 miRNAs and their expression trends were similar to the results obtained by Solexa sequencing. Further, a total of 89 target genes for conserved and 26 target genes for novel miRNAs were predicted. This study will help in understanding the roles and regulatory modes of miRNAs involvement in the hickory graft process.