A Magnetic Nanoparticle-Based Time-Resolved Fluoroimmunoassay for Determination of the Cytokeratin 19 Fragment in Human Serum

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• 作者：Guanfeng Lin ; Tiancai Liu ; Jingyuan Hou ; Zhiqi Ren ; Jianwei Zhou…
• 关键词：Magnetic nanoparticles ; Time ; resolved fluoroimmunoassay ; Cytokeratin 19 fragment
• 刊名：Journal of Fluorescence
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：25
• 期：2
• 页码：361-367
• 全文大小：383 KB
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  17. Chapman, MH, Sand
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Biophysics and Biomedical Physics
  Biotechnology
  Biochemistry
  Analytical Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4994

文摘

A sensitive, rapid and novel measurement method for cytokeratin 19 fragment (CYFRA 21-) in human serum by magnetic particle-based time-resolved fluoroimmunoassay (TRFIA) is described. Built on a sandwich-type immunoassay format, analytes in samples were captured by one monoclonal antibody coating onto the surface of magnetic beads and “sandwiched-by another monoclonal antibody labeled with europium chelates. The coefficient variations of the method were lower than 7?%, and the recoveries were in the range of 90-10?% for serum samples. The lower limit of quantitation of the present method for CYFRA 21- was 0.78?ng/ml. The correlation coefficient of CYFRA 21- values obtained by our novel TRFIA and CLIA was 0.980. The present novel TRFIA demonstrated high sensitivity, wider effective detection range and excellent reproducibility for determination of CYFRA 21- can be useful for early screening and prognosis evaluation of patients with non-small cell lung cancer.