Induction of 2n pollen with colchicine during microsporogenesis in Eucalyptus

详细信息    查看全文

• 作者：Jun Yang ; Pengqiang Yao ; Yun Li ; Jiyou Mo ; Jianzhong Wang ; Xiangyang Kang
• 刊名：Euphytica
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：210
• 期：1
• 页码：69-78
• 全文大小：960 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5060
• 卷排序：210

文摘

Artificially inducing 2n gametes through chromosome doubling is an effective way to obtain polyploids. In this study, Eucalyptus urophylla microsporogenesis and flower development were investigated to guide 2n pollen induction. We also investigated suitable conditions for colchicine treatment. Our results showed that E. urophylla 2n pollen was spherical and had a large volume (mean diameter 28.57 ± 0.46 μm), while normal untreated pollen (mean diameter 19.68 ± 0.11 μm) was tetrahedron. The highest rate of 2n pollen production was 28.71 % when the flower buds, which ranged in size from 3.5 to 4.0 mm, underwent treatment with 0.5 % colchicine solution for 6 h. Further studies suggested that diplotene to diakinesis and metaphase I to telophase I were suitable meiotic stages for chromosomes doubling, due to asynchronous development of microsporogenesis between the anthers in a single flower bud. These data help to illuminate research in other areas, such as triploid eucalyptus production by chromosome doubling of female gametes.Keywords2n PollenChromosome doublingColchicineEucalyptusMicrosporogenesis