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Design and preparation of quantum dots fluorescent probes for in situ identification of Microthrix parvicella in bulking sludge
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  • 作者：Xuening Fei ; Wenke Sun ; Lingyun Cao ; Xiumei Jiao…
  • 关键词：Fluorescent QDs probes ; Hydrophobic ; In situ identification ; Microthrix parvicella
  • 刊名：Applied Microbiology and Biotechnology
  • 出版年：2016
  • 出版时间：January 2016
  • 年：2016
  • 卷：100
  • 期：2
  • 页码：961-968
  • 全文大小：1,325 KB
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  • 作者单位：Xuening Fei (1) (2) (3)
    Wenke Sun (1)
    Lingyun Cao (2)
    Xiumei Jiao (3)
    Dayong Lin (3)
    Guozhi Jia (2)

    1. School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, 300384, China
    2. School of Science, Tianjin Chengjian University, No. 26 Jinjing Road, Xiqing District, Tianjin, 300384, China
    3. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China
  • 刊物类别：Chemistry and Materials Science
  • 刊物主题：Chemistry
    Biotechnology
    Microbiology
    Microbial Genetics and Genomics
  • 出版者：Springer Berlin / Heidelberg
  • ISSN：1432-0614
文摘
A series of quantum dots (QDs) fluorescent probes for the in situ identification of Microthrix parvicella (M. parvicella) in bulking sludge were designed and prepared. In the preparation of CdTe/CdS QDs, the 11-mercaptoundecanoic acid (11-acid) and 16-mercaptohexadecanoic acid (16-acid) were used as the stabilizer. The prepared QDs probes were characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), and transmission electron microscopy (TEM), and the results showed that the CdTe/CdS QDs formed a core-shell structure and the long carbon chain was successfully grafted onto its surface. And the three QDs probes had different crystallinity and particle size, which was due to the inhibition effect of long carbon chain. The optical properties test results showed that although the formed core-shell structure and long carbon chain affected the fluorescent intensity, adsorption, and emission spectra of the QDs probes, the probes B and C had a large stokes-shift of 82 and 101 nm, which was a benefit for their fluorescent labeling property. In the fluorescent identification of M. parvicella, the probes B and C effectively adsorbed onto the surface of M. parvicella through a hydrophobic bond, and then identified M. parvicella by their unique fluorescence. In addition, it was found that a better hydrophobic property resulted in better identification efficiency. Keywords Fluorescent QDs probes Hydrophobic In situ identification Microthrix parvicella

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