The Protective Roles of Selenium on Hepatic Tissue Ultrastructure and Mitochondrial Antioxidant Capacity in Copper-Overloaded Rats

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• 作者：Rongsheng Su ; Huabin Cao ; Jiaqiang Pan ; Chengmei Li…
• 关键词：Sodium selenite ; Copper ; Toxicity ; Ultrastructure ; Hepatic mitochondria
• 刊名：Biological Trace Element Research
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：167
• 期：1
• 页码：110-114
• 全文大小：260 KB
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• 作者单位：Rongsheng Su (1)
  Huabin Cao (2)
  Jiaqiang Pan (1)
  Chengmei Li (1)
  Yizhou Chen (1)
  Zhaoxin Tang (1)
  
  1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China
  2. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China
  
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720

文摘

The aim of this study was to explore the effects of selenium addition on hepatic tissue ultrastructure and mitochondrial antioxidant capacity in copper-overloaded rats. Eighteen adult male Sprague-Dawley (SD) rats were randomly divided into three groups (n-- per group). Each group received 1?mL (intragastrically) of water (control, group I) or water containing copper chloride (CuCl2, 1?mol/L) (group II) or a mixture of CuCl2 (1?mol/L) with sodium selenite (Na2SeO3, 0.05?mol/L) (group III) once daily for 30?days. Histological examination revealed normal hepatocyte structure and no ultrastructural changes in mitochondria in controls. In contrast, group II exhibited severe ultrastructural alterations, fuzzy mitochondrial membranes, irregularly shaped and fragmented rough endoplasmic reticulum (RER), and the high melanin content; group III also exhibited larger amounts of engulfing vesicles (EV) in the cytoplasm. Compared to controls, the CuCl2 treatment lowered (P-lt;-.05) hepatocyte enzymatic activities and increased lipid peroxidation as indicated by an increase in malondialdehyde (MDA) levels. The effects of CuCl2 were attenuated by simultaneous administration with Na2SeO3. These results indicated that the adverse effects of copper toxicity can be partially attenuated by providing a source of selenium.