Alterations in Membrane Phospholipid Fatty Acids of Gram-Positive Piezotolerant Bacterium Sporosarcina sp. DSK25 in Response to Growth Pressure
详细信息 查看全文
- 作者：Jiani Wang (1)
Jiangtao Li (1)
Shamik Dasgupta (1)
Li Zhang (2)
Mikhail Y. Golovko (3)
Svetlana A. Golovko (3)
Jiasong Fang (1) (4) - 关键词：Adaptation ; Gram ; positive bacteria ; Growth pressure ; Phospholipid fatty acids ; Piezophilic bacteria ; Sporosarcina sp. DSK25
- 刊名：Lipids
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：49
- 期：4
- 页码：347-356
- 全文大小：554 KB
- 参考文献：1. Bartlett DH, Welch TJ (1995) / ompH gene expression is regulated by multiple environmental cues in addition to high pressure in the deep-sea bacterium / Photobacterium species strain SS9. J Bacteriol 177:1008-016
2. Whitman WB, Coleman DC, Wiebe WJ (1998) Prokaryotes-the unseen majority. Proc Natl Acad Sci USA 95:6578-583 CrossRef
3. Bartlett DH (2008) Introduction to deep sea microbiology. In: Michiels C, Bartlett DH, Aertsen A (eds) High-pressure microbiology. American Society for Microbiology, Washington, D.C., pp 219-64
4. Yayanos AA, Diets AS, Van Boxtel R (1979) Isolation of a deep-sea barophilic bacterium and some of its growth characteristics. Science 205:808-10 CrossRef
5. Cossins AR, Sinensky M (1984) Adaptation of membranes to temperature, pressure and exogenous lipids. In: Shinitzky M (ed) Physiology of membrane fluidity, vol 2. CRC Press, Boca Raton, pp 1-6
6. Cronan JE Jr, Waldrop LG (2002) Multi-subunit acetyl-CoA carboxylases. Prog Lipid Res 41:407-35 CrossRef
7. Klein W, Weber MHW, Marahiel MA (1999) Cold shock response of / Bacillus subtilis: isoleucine-dependent switch in the fatty acid branching pattern for membrane adaptation to low temperatures. J Bacteriol 181:5341-349
8. Yamanaka K (1999) Cold shock response in / Escherichia coli. J Mol Microbiol Biotechnol 1:193-02
9. Weber MHW, Klein W, Müller L, Niess UM, Marahiel MA (2001) Role of the / Bacillus subtilis fatty acid desaturase in membrane adaptation during cold shock. Mol Microbiol 39:1321-329 CrossRef
10. Weber MHW, Marahiel MA (2002) Coping with the cold: the cold shock response in the Gram-positive soil bacterium / Bacillus subtilis. Phil Trans R Soc Lond B 357:895-07 CrossRef
11. Mansilla MC, Aguilar PS, Albanesi D, Cybulski LE, Altabe S, de Mendoza D (2003) Regulation of fatty acid desaturation in / Bacillus subtilis. Prostaglandins Leukot Essent Fatty Acids 68:187-90 CrossRef
12. Sinensky M (1974) Homoviscous adaptation––a homeostatic process that regulates the viscosity of membrane lipids in / Escherichia coli. Proc Natl Acad Sci USA 71:522-25 CrossRef
13. Russell NJ (1984) Mechanisms of the thermal adaptation in bacteria: blueprints for survival. Trends Biochem Sci 9:108-12 CrossRef
14. Kaneda T (1991) / Iso- and / anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol Rev 55:288-02
15. de Mendoza D, Grau R, Cronan JE Jr (1993) Biosynthesis and function of membrane lipids. In: Losick R, Sonenshein AL, Hoch JA (eds) / Bacillus subtilis and other Gram-positive bacteria: physiology, biochemistry, and molecular biology. American Society for Microbiology, Washington, pp 411-25
16. Morita RY (1986) Pressure as an extreme environment. In: Herbert RA, Codd GA (eds) Microbes in extreme environments. Academic, London, pp 171-85
17. DeLong EF, Yayanos AA (1985) Adaptation of membrane lipids of a deep-sea bacterium to changes in hydrostatic pressure. Science 228:1101-103 CrossRef
18. Allen EE, Facciotti D, Bartlett HD (1999) Monounsaturated but not polyunsaturated fatty acids are required for growth of the deep-sea bacterium / Photobacterium profundum SS9 at high pressure and low temperature. Appl Environ Microbiol 66:1710-720
19. Melchior DL (1982) Lipid phase transitions and regulation of membrane fluidity in prokaryotes. Curr Top Membr Transp 17:263-07 CrossRef
20. Russell NJ (1988) Functions of lipids: structural roles and membrane functions. In: Ratledge C, Wilkinson SG (eds) Microbial lipids, vol 2. Academic, London, pp 279-65
21. Fang J, Kato C, Sato T, Chan O, McKay D (2004) Biosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria. Comp Biochem Physiol B 137:455-61 CrossRef
22. Kamimura K, Fuse H, Takimura O, Yamaoka Y (1993) Effects of growth pressure and temperature on fatty acid composition of a barotolerant deep-sea bacterium. Appl Environ Microbiol 59:924-26
23. Lauro FM, Bartlett D (2007) Prokaryotic lifestyles in deep sea habitats. Extremophiles 12:15-5 CrossRef
24. Kato C, Suzuki S, Hata S, Ito T, Horikoshi K (1995) The properties of a protease activated by high pressure from / Sporosarcina sp. strain DSK25 isolated from deep-sea sediment. JAMSTECR 32:7-2
25. Kato C, Qureshi HM (1999) Pressure response in deep-sea piezophilic bacteria. J Molec Microbial Biotechnol 1:87-2
26. Fang J, Uhle M, Billmark K, Bartlett DH, Kato C (2006) Fractionation of carbon isotopes in biosynthesis of fatty acids by a piezophilic bacterium / Moritella japonica strain DSK1. Geochim Cosmochim Acta 70:1753-760 CrossRef
27. Fang J, Findlay RH (1996) The use of a classic lipid extraction method for simultaneous recovery of organic pollutants and microbial lipids from sediments. J Microbiol Methods 27:63-1 CrossRef
28. Fang J, Gupta SD, Hasiotis ST, Brake SS, Bazylinski DA (2007) Microbial community structure of a stromatolite from an acid mine drainage system, implications for the role of microeukaryotes in the formation of ancient Fe-rich stromatolites. Chem Geol 243:191-04 CrossRef
29. Fang J, Chan O, Kato C, Sato T, Peeples T, Niggemeyer K (2003) Phospholipid fatty acid profiles of piezophilic bacteria from the deep sea. Lipids 38:885-87 CrossRef
30. Sikorski J, Brambilla E, Kroppenstedt RM, Tindall BJ (2008) The temperature adaptive fatty acid content in / Bacillus simplex strains from “Evolution Canyon- Israel Microbiol 154:2416-426 CrossRef
31. Brose S, Baker A, Golovko M (2013) A fast one-step extraction and UPLC–MS/MS analysis for E2/D2 series prostaglandins and isoprostanes. Lipids 48:411-19 CrossRef
32. MacDonald AG (1984) The effects of pressure on the molecular structure and physiological function of cell membranes. Phil Trans R Soc Lond 304B:47-8 CrossRef
33. Aguilar PS, Cronan JE Jr, de Mendoza D (1998) A / Bacillus subtilis gene induced by cold shock encodes a membrane phospholipid desaturase. J Bacteriol 180:2194-200
34. Lopez MM, Makhatadze GI (2000) Major cold shock proteins, CspA from / Escherichia coli and CspB from / Bacillus subtilis, interact differently with single-stranded DNA templates. Biochim Biophys Acta 1479:196-02 CrossRef
35. Aguilar PS, Hernandez-Arriaga AM, Cybulski LE, Erazo AC, de Mendoza D (2001) Molecular basis of thermosensing: a two-component signal transduction thermometer in / Bacillus subtilis. EMBO J 20:1681-691 CrossRef
36. Aguilar PS, Lopez P, de Mendoza D (1999) Transcriptional control of the low-temperature-inducible des gene, encoding the Δ5 desaturase of / Bacillus subtilis. J Bacteriol 181:7028-033
37. Metz JG, Roessler P, Facciotti D, Leverine C, Dittrich F, Lassner M, Valentine R, Lardizabal K, Domergue F, Yamada A, Yazawa K, Knauf V, Browse J (2001) Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science 293:290-93 CrossRef
38. Fang J, Kato C (2007) The use of lipid biomarkers and stable carbon isotopes to characterize deep-sea piezophilic bacteria. In: Méndez-Vilas A (ed) Communicating current research and educational topics and trends in applied microbiology. Formatex Center, Spain, pp 190-00 - 作者单位：Jiani Wang (1)
Jiangtao Li (1)
Shamik Dasgupta (1)
Li Zhang (2)
Mikhail Y. Golovko (3)
Svetlana A. Golovko (3)
Jiasong Fang (1) (4)
1. State Key Laboratory of Marine Geology, Tongji University, Shanghai, People’s Republic of China
2. State Key Laboratory of Geological Processes and Mineral Resources, Faculty of Earth Sciences, China University of Geosciences, Wuhan, 430074, People’s Republic of China
3. Department of Basic Sciences, University of North Dakota, Grand Forks, ND, 58202, USA
4. Department of Natural Sciences, Hawaii Pacific University, Kaneohe, HI, 96744, USA - ISSN：1558-9307
文摘
Pressure is an important thermodynamic property of the ocean and the deep biosphere that affects microbial physiology and biochemistry. Here, we report on our investigation of the response of Gram-positive piezotolerant bacterium Sporosarcina sp. DSK25 to hydrostatic pressure. Strain DSK25 responded in an adaptive manner to upshifts of growth pressure and showed systematic changes in phospholipid fatty acids. As the pressure increased from 0.1 to 10?MPa (Megapascal), unsaturated fatty acids in DSK25 increased from 21.7 to 31.1?% of total fatty acids, while the level of iso- and anteiso-branched fatty acids remained unchanged. At higher pressures (30, 50, and 60?MPa), the amount of unsaturated fatty acids decreased, and that of anteiso-branched fatty acids increased from 34.4 to 49.9?% at the expense of iso-branched fatty acids. For the first time, two polyunsaturated fatty acids (PUFA), 18:2n-6 and 18:2n-x, with the latter having much higher abundance than the former, were identified in DSK25. The concentration of the PUFA increased with growth pressure. These results indicate the involvement of unsaturated and methyl-branched fatty acids in the modulation of bacteria membrane fluidity and function over environmentally relevant parameter (pressure). Piezotolerant bacterium Sporosarcina sp. DSK25 appears to utilize two regulatory mechanisms for adaptation to high pressure, a rapid-responding mechanism on transient scale, expressed as increased biosynthesis of monounsaturated fatty acids, and a long-term adaptation mechanism in increased synthesis of anteiso-branched and polyunsaturated fatty acids. Our results further suggest that Gram-positive piezophilic bacteria respond differently than Gram-negative bacteria in adaptation to high pressure.