GDNF and NT-3 Induce Progenitor Bone Mesenchymal Stem Cell Differentiation into Neurons in Fetal Gut Culture Medium

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• 作者：Tianqi Zhu (1)
  Donghai Yu (1)
  Jiexiong Feng (1)
  Xiaojuan Wu (1)
  Lei Xiang (1)
  Heyun Gao (1)
  Xueqin Zhang (1)
  Mingfa Wei (1)
  
  1. Department of Pediatric Surgery ; Tongji Hospital ; Huazhong University of Science and Technology ; 1095 Jiefang Avenue ; Hankou ; Wuhan ; China
  
• 关键词：Bone mesenchymal stem cell differentiation ; Glial cell ; derived neurotrophic factor (GDNF) ; Neurotrophin ; 3 (NT ; 3) ; Electrophysiology ; Cell therapy
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：35
• 期：2
• 页码：255-264
• 全文大小：1,623 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830

文摘

With the increasing use of bone marrow mesenchymal stem cells (BMSCs) in cell therapies, factors regulating BMSC differentiation have become the interest of current research. In this study, we investigated the effects of glial cell-derived neurotrophic factor (GDNF) and neurotrophin-3 (NT-3) on the course of BMSC differentiation. BMSCs were isolated from rat bone marrow and transfected with GDNF and NT-3 genes. Compared to mock-transfected BMSCs, GDNF and NT-3 induced BMSC differentiation to reveal neuron-like characteristics, i.e., the positive expression of neuronal marker MAP-2 and astrocyte marker GFAP, as detected by immunofluorescence assays. Semi-quantitative polymerase chain reaction (PCR) and western blot analyses showed that the increase of expression of GDNF and NT-3 in BMSCs also simultaneously elevated the mRNA expression of NSE, nestin, and MAP-2. Furthermore, the cell patch-clamp test demonstrated that the overexpression of GDNF and NT-3 in BMSCs enhanced voltage-activated potassium currents, implying that BMSCs possess great potential as a cell-based therapeutic candidate to treat neurological diseases.