Identification of Rat Respiratory Mucosa Stem Cells and Comparison of the Early Neural Differentiation Potential with the Bone Marrow Mesenchymal Stem Cells In Vitro
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- 作者：Xin Gao (1)
Jian Zhang (1)
Jun Zhang (1)
Hongjun Zou (1)
Jinbo Liu (1) - 关键词：Respiratory mucosa ; mesenchymal stem cells ; Bone marrow mesenchymal stem cells ; Neural ; differentiation ; Neural cells
- 刊名：Cellular and Molecular Neurobiology
- 出版年：2014
- 出版时间：March 2014
- 年：2014
- 卷：34
- 期：2
- 页码：257-268
- 全文大小：1,295 KB
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Jian Zhang (1)
Jun Zhang (1)
Hongjun Zou (1)
Jinbo Liu (1)
1. Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou, 213003, Jiangsu, China - ISSN：1573-6830
文摘
The aim of this study is to identify rat nasal septum respiratory mucosa-derived mesenchyme stem cells (RM-MSCs) and to compare its neural lineage differentiation capacity with bone marrow-derived mesenchyme stem cells (BM-MSCs) after a short period of neural induction culture in vitro. The cell morphology was observed with light microscopy; cell proliferation was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The characteristics of the cells were evaluated with flow cytometry, immunofluorescence, real-time quantitative PCR (RT-PCR), and Western blotting. The results showed that rat nasal respiratory mucosa contains RM-MSCs that exhibited similar proliferation rate as BM-MSCs in vitro. Both RT-PCR and Western blotting analyses demonstrated that RM-MSCs showed higher expression of neural lineage markers than BM-MSCs after a short period of neural induction culture, and secreted higher level of brain-derived neurotrophic factor. RM-MSCs were more amenable to differentiate into neural or glial cell after a short period of neural induction culture than BM-MSCs in vitro; and it could be considered as another optimal source of stem cells for cell-based therapy to neurological diseases.