Expression of Peroxiredoxin 1 After Traumatic Spinal Cord Injury in Rats

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• 作者：Shen Huang ; Xiaojuan Liu ; Jinlong Zhang ; Guofeng Bao&#8230
• 关键词：Spinal cord injury (SCI) ; Peroxiredoxin 1 (PRDX1) ; Glial cell proliferation ; Rats
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：35
• 期：8
• 页码：1217-1226
• 全文大小：3,633 KB
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• 作者单位：Shen Huang (1) (5)
  Xiaojuan Liu (2) (5)
  Jinlong Zhang (1) (5)
  Guofeng Bao (1)
  Guanhua Xu (1)
  Yuyu Sun (1)
  Qijie Shen (3) (5)
  Min Lian (3) (5)
  Yuwei Huang (4) (5)
  Zhiming Cui (1) (5)
  
  1. Department of Spine Surgery, The Second Affiliated Hospital, Nantong University, Nantong, 226001, People鈥檚 Republic of China
  5. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College, Nantong University, Nantong, Jiangsu, 226001, People鈥檚 Republic of China
  2. Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People鈥檚 Republic of China
  3. Department of Stomatology, Affiliated Hospital of Nantong University, Nantong, 226001, People鈥檚 Republic of China
  4. Institute of Navigation Medicine, Nantong University, Nantong, Jiangsu, 226001, People鈥檚 Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830

文摘

Reactive astrogliosis and microgliosis after spinal cord injury (SCI) contribute to glial scar formation that impedes axonal regeneration. The mechanisms underlying reactive astrocyte and microglia proliferation upon injury remain partially understood. Peroxiredoxin 1 (PRDX1) is an antioxidant participating in cell proliferation, differentiation, and apoptosis. However, PRDX1 functions in SCI-induced astrocyte and microglia proliferation are unknown. In this study, we established an acute spinal cord contusion injury model in adult rats to investigate the potential role of PRDX1 during the pathological process of SCI. We found the palpable expression increase of PRDX1 after SCI by western blot and immunohistochemistry staining. Double immunofluorescence staining showed that PRDX1 expression mainly increased in astrocytes and microglia. In addition, PRDX1/proliferating cell nuclear antigen (PCNA) colocalized in astrocytes and microglia. Furthermore, PCNA expression also elevated after SCI, as well as was positively correlated with PRDX1 expression. In vitro, PRDX1 expression in primary rat spinal cord astrocytes and microglia changed in a concentration- and time-dependent manner according to LPS treatment. In addition, PRDX1 knockdown in astrocytes and microglia resulted in the decrease of PCNA expression after LPS stimulation, showing that PRDX1 promoted astrocyte and microglia proliferation after inflammation. Our results suggested that PRDX1 might play a crucial role in astrocyte and microglia proliferation after SCI. Keywords Spinal cord injury (SCI) Peroxiredoxin 1 (PRDX1) Glial cell proliferation Rats