Genotypic Analyses and Virulence Characterization of Listeria monocytogenes Isolates from Crayfish (Procambarus clarkii)

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• 作者：Jinquan Li (1) (2)
  Pujun Du (1)
  Zhi Li (1)
  Yang Zhou (3)
  Wei Cheng (4)
  Si Wu (1)
  Fusheng Chen (1) (2)
  Xiaohong Wang (1) (2)
  
  1. Key Laboratory of Environment Correlative Dietology ; College of Food Science and Technology ; Huazhong Agricultural University ; Wuhan ; 430070 ; Hubei ; People鈥檚 Republic of China
  2. State Key Laboratory of Agricultural Microbiology ; Huazhong Agricultural University ; Wuhan ; 430070 ; Hubei ; People鈥檚 Republic of China
  3. College of Fisheries ; Huazhong Agricultural University ; Wuhan ; 430070 ; Hubei ; People鈥檚 Republic of China
  4. Xiantao Entry Exit Inspection and Quarantine Bureau ; Xiantao ; People鈥檚 Republic of China
  
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：70
• 期：5
• 页码：704-709
• 全文大小：397 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Listeria monocytogenes is a foodborne pathogen that can cause invasive illness in humans and farm animals. It is frequently isolated from dairy products and poultry. However, there have been few literatures on the genetic diversity and virulence potential of L. monocytogenes from freshwater animal. Thirty-nine L. monocytogenes strains from crayfish were isolated and identified in this study. Molecular subtyping and polymorphism of each isolate were analyzed by multilocus sequence typing (MLST). MLST divided the isolates into eight sequence types (STs), six of which from crayfish were the same with the isolates from environment and clinic. PCR detection of the eight genes related to virulence and multiplex PCR for serotyping showed that the eight virulence factors were present in the isolates and all the isolates belonged to four major L. monocytogenes serotype groups (1/2a, 1/2b, 1/2c, and 4b) frequently isolated from patients. In vivo pathogenicity of isolates was also evaluated in murine model and survival curve of infected mice suggested that ST1, ST4, and ST9 isolates were as virulent as the reference strain EGDe. This study provides preliminary insights into the genetic diversity of L. monocytogenes from crayfish and the genetic correlation between crayfish and clinical L. monocytogenes isolates. The results indicate the contamination in aquaculture could be the source of Listeria contamination and the isolates are likely to cause human listeriosis.