A high accuracy cantilever array sensor for early liver cancer diagnosis
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- 作者：Jingjing Wang ; Lihao Wang ; Yinfang Zhu ; Jinying Zhang ; Ju Liao…
- 关键词：MEMS cantilever ; Mass detection ; Stiffness coefficient ; Liver cancer ; Multiple biomarkers
- 刊名：Biomedical Microdevices
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：18
- 期：6
- 全文大小：1,978 KB
- 刊物类别：Engineering
- 刊物主题：Biomedical Engineering
Biophysics and Biomedical Physics
Nanotechnology
Engineering Fluid Dynamics - 出版者：Springer Netherlands
- ISSN：1572-8781
- 卷排序：18
文摘
Early liver cancer diagnosis has clinical significance in treating cancer. Joint detection of multiple biomarkers has been considered as an effective and reliable method for early cancer diagnosis. In this work, a novel biosensor based on microcantilever array was batch-fabricated for multiple liver cancer biomarkers detection with high sensitivity, high accuracy, high throughput, and high specification. A micro-cavity was designed in the free end of the cantilever for local reaction between antibody and antigen, which can dramatically reduce the effect of adsorption-induced stiffness coefficient k variation. Furthermore,the pillar arrays in the micro-cavity were designed for increasing detection upper limit. A linear relationship between the relative frequency shift and the antigen concentration was observed for three liver cancer biomarkers, alpha-fetoprotein (AFP), γ-glutamyltranspeptidase II (GGT-2), and hepatocyte growth factor (HGF). Slight cross-reaction response to different antigens ensures high specificity of the sensor. These features will promote clinical application of the cantilever sensors in early cancer diagnosis.