The effect of CXCL9 on the invasion ability of hepatocellular carcinoma through up-regulation of PREX2

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• 作者：Xiaoqin Lan (1) (2)
  Fang Xiao (1)
  Qiang Ding (1)
  Jiqiao Liu (1)
  Jingmei Liu (1)
  Junhua Li (3)
  Jiong Zhang (3)
  De-An Tian (1)
  
• 关键词：CXCL9 ; PREX2 ; Liver cancer cell lines ; Invasion ; Metastasis
• 刊名：Journal of Molecular Histology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：45
• 期：6
• 页码：689-696
• 全文大小：911 KB
• 参考文献：1. Anson M, Crain-Denoyelle AM, Baud V, Chereau F, Gougelet A, Terris B, Yamagoe S, Colnot S, Viguier M, Perret C, Couty JP (2012) Oncogenic beta-catenin triggers an inflammatory response that determines the aggressiveness of hepatocellular carcinoma in mice. J Clin Invest 122:586-99 lank" title="It opens in new window">CrossRef
  2. Barrio-Real L, Kazanietz MG (2012) Rho GEFs and cancer: linking gene expression and metastatic dissemination. Sci Signal 5:pe43 lank" title="It opens in new window">CrossRef
  3. Ben-Neriah Y, Karin M (2011) Inflammation meets cancer, with NF-kappaB as the matchmaker. Nat Immunol 12:715-23 lank" title="It opens in new window">CrossRef
  4. Berger MF, Hodis E, Heffernan TP, Deribe YL, Lawrence MS, Protopopov A, Ivanova E, Watson IR, Nickerson E, Ghosh P, Zhang H, Zeid R, Ren X, Cibulskis K, Sivachenko AY, Wagle N, Sucker A, Sougnez C, Onofrio R, Ambrogio L, Auclair D, Fennell T, Carter SL, Drier Y, Stojanov P, Singer MA, Voet D, Jing R, Saksena G, Barretina J, Ramos AH, Pugh TJ, Stransky N, Parkin M, Winckler W, Mahan S, Ardlie K, Baldwin J, Wargo J, Schadendorf D, Meyerson M, Gabriel SB, Golub TR, Wagner SN, Lander ES, Getz G, Chin L, Garraway LA (2012) Melanoma genome sequencing reveals frequent PREX2 mutations. Nature 485:502-06
  5. Chen X, Pan M, Han L, Lu H, Hao X, Dong Q (2013) miR-338-3p suppresses neuroblastoma proliferation, invasion and migration through targeting PREX2a. FEBS Lett 587:3729-737 lank" title="It opens in new window">CrossRef
  6. Cui X, Liu Y, Wan C, Lu C, Cai J, He S, Ni T, Zhu J, Wei L, Zhang Y, Qian H (2014) Decreased expression of SERPINB1 correlates with tumor invasion and poor prognosis in hepatocellular carcinoma. J Mol Histol 45:59-8 lank" title="It opens in new window">CrossRef
  7. de Martel C, Ferlay J, Franceschi S, Vignat J, Bray F, Forman D, Plummer M (2012) Global burden of cancers attributable to infections in 2008: a review and synthetic analysis. Lancet Oncol 13:607-15 lank" title="It opens in new window">CrossRef
  8. Donald S, Hill K, Lecureuil C, Barnouin R, Krugmann S, John Coadwell W, Andrews SR, Walker SA, Hawkins PT, Stephens LR, Welch HC (2004) P-Rex2, a new guanine-nucleotide exchange factor for Rac. FEBS Lett 572:172-76 lank" title="It opens in new window">CrossRef
  9. El-Serag HB, Rudolph KL (2007) Hepatocellular carcinoma: epidemiology and molecular carcinogenesis. Gastroenterology 132:2557-576 lank" title="It opens in new window">CrossRef
  10. Fernandez-Zapico ME, Gonzalez-Paz NC, Weiss E, Savoy DN, Molina JR, Fonseca R, Smyrk TC, Chari ST, Urrutia R, Billadeau DD (2005) Ectopic expression of VAV1 reveals an unexpected role in pancreatic cancer tumorigenesis. Cancer Cell 7:39-9 lank" title="It opens in new window">CrossRef
  11. Franceschi S, Montella M, Polesel J, La Vecchia C, Crispo A, Dal Maso L, Casarin P, Izzo F, Tommasi LG, Chemin I, Trepo C, Crovatto M, Talamini R (2006) Hepatitis viruses, alcohol, and tobacco in the etiology of hepatocellular carcinoma in Italy. Cancer Epidemiol Biomarkers Prev 15:683-89 lank" title="It opens in new window">CrossRef
  12. Ganem D, Prince AM (2004) Hepatitis B virus infection–natural history and clinical consequences. N Engl J Med 350:1118-129 lank" title="It opens in new window">CrossRef
  13. Gilmore TD (2003) The Re1/NF-kappa B/I kappa B signal transduction pathway and cancer. Cancer Treat Res 115:241-65 lank" title="It opens in new window">CrossRef
  14. Hernandez L, Hsu SC, Davidson B, Birrer MJ, Kohn EC, Annunziata CM (2010) Activation of NF-kappaB signaling by inhibitor of NF-kappaB kinase beta increases aggressiveness of ovarian cancer. Cancer Res 70:4005-014 lank" title="It opens in new window">CrossRef
  15. Li J, Lau G, Chen L, Yuan YF, Huang J, Luk JM, Xie D, Guan XY (2012) Interleukin 23 promotes hepatocellular carcinoma metastasis
• 作者单位：Xiaoqin Lan (1) (2)
  Fang Xiao (1)
  Qiang Ding (1)
  Jiqiao Liu (1)
  Jingmei Liu (1)
  Junhua Li (3)
  Jiong Zhang (3)
  De-An Tian (1)
  
  1. Department of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
  2. Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China
  3. Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
  
• ISSN：1567-2387

文摘

Elevated expression of CXCL9 has been shown to involve in the infiltration of inflammatory cells and liver damage after Hepatitis B virus (HBV) infection. However, whether and by what underlying mechanism does CXCL9 play a role in HBV infection associated hepatocellular?carcinoma (HCC) invasion ability remain unclear. In this study, human HCC as well as adjacent noncancerous tissues, together with three kinds of liver cancer cell lines were investigated to clarify the possible involvement of CXCL9 in the regulation of HCC invasion and metastasis. Invasion ability of liver cancer cells were evaluated by transwell assays and it is enhanced after co-cultured with recombined human CXCL9 (rhCXCL9). As a trigger of Rac GTPase signaling after G protein-coupled receptors (GPCR) activated by CXCL9, Phosphatidylinositol-3, 4, 5-trisphosphate RAC Exchanger 2 (PREX2) mRNA expression of the liver cancer cell lines was elevated after co-cultured with rhCXCL9. Moreover, the mRNA level of PREX2 in HCC tissues was significantly higher than those in adjacent noncancerous tissues. Besides, the mRNA levels of PREX2 were positively correlated with the poor differentiation, portal vein invasion, metastasis and qualitative HbsAg results in 45 pairs of HCC specimens. Similarly, PREX2 mRNA was higher in three liver cancer cell lines when compared with the normal liver cell line whereas knocked down of PREX2 by small interference RNA (PREX2-siRNA) reduced the invasion ability of liver cancer cells in transwell assays. Overall, our results suggested CXCL9 was involved in the invasion ability of HCC possibly through up-regulation of its potential effector PREX2.