Cloning of catalase and expression patterns of catalase and selenium-dependent glutathione peroxidase from Exopalaemon carinicauda in response to low salinity stress

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• 作者：Hai Ren ; Jian Li ; Jitao Li ; Yu Ying ; Hongxing Ge ; Dongli Li…
• 关键词：Exopalaemon carinicauda ; catalase (CAT) ; selenium ; dependent glutathione peroxidase (Se ; GPx) ; cloning ; expression
• 刊名：Acta Oceanologica Sinica
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：34
• 期：8
• 页码：52-61
• 全文大小：1,454 KB
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  González-Rodríguez G, Colubi
• 作者单位：Hai Ren (1) (2)
  Jian Li (2)
  Jitao Li (2)
  Yu Ying (2)
  Hongxing Ge (2)
  Dongli Li (2)
  Tianji Yu (2)
  
  1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China
  2. Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China
  
• 刊物主题：Oceanography; Climatology; Ecology; Engineering Fluid Dynamics; Marine & Freshwater Sciences; Environmental Chemistry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-1099

文摘

Catalase (CAT) and selenium-dependent glutathione peroxidase (Se-GPx) play a vital role in protecting organisms against various oxidative stresses by eliminating H2O2. The objective of this paper is to evaluate the roles of these antioxidant molecules in the ridgetail white prawn Exopalaemon carinicauda in response to low salinity stress. A complementary DNA (cDNA) containing the complete coding sequence of CAT was cloned from the hepatopancreas using reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The full-length cDNA of CAT (2 649 bp) contains a 5-untranslated region (UTR) of 78 bp, a 3-UTR of 1 017 bp, with a poly (A) tail, and an open reading frame of 1 554 bp encoding a 517-amino-acid polypeptide with predicted molecular mass of 58.46 kDa and estimated isoelectric point of 6.64. This CAT sequence contained the proximal active site signature (60FDRERIPERVVHAKGAG76), proximal heme-ligand signature sequence (350RLFSYPDTH358) and three catalytic amino acid residues (His71, Asn144 and Tyr354). Sequence comparison showed that the CAT deduced amino acid sequence of E. carinicauda shared 68%-92% of identities with those of other species. Quantitative real-time PCR analysis revealed that CAT mRNA was widely expressed in the hepatopancreas (highest), hemocyte, eyestalk, heart, gill, muscle, ovary and stomach. Under low salinity stress, CAT and GPx mRNA expression levels both in the gill and hepatopancreas increased significantly at the first 48 h and 6 h respectively, indicating a tissue- and time-dependent antioxidant response in E. carinicauda. All these results indicate that E. carinicauda CAT is a member of the CAT family and might be involved in the acute response against low salinity stress.