Changes in Surface Charge Density of Blood Cells in Fatal Accidental Hypothermia

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• 作者：Micha? Szeremeta ; Aneta Dorota Petelska ; Joanna Kotyńska…
• 关键词：Fatal accidental hypothermia ; Surface charge density ; pH measurement ; Erythrocyte ; Thrombocyte
• 刊名：Journal of Membrane Biology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：248
• 期：6
• 页码：1175-1180
• 全文大小：413 KB
• 参考文献：Alexander AE, Johnson P (1949) Colloid science. Clarendon Press, Oxford
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• 作者单位：Micha? Szeremeta (1)
  Aneta Dorota Petelska (2)
  Joanna Kotyńska (2)
  Witold Pepiński (1)
  Monika Naumowicz (2)
  Zbigniew Artur Figaszewski (2) (3)
  Anna Niemcunowicz-Janica (1)
  
  1. Department of Forensic Medicine, Medical University of Bialystok, Waszyngtona St. 13, 15-230, Bialystok, Poland
  2. Institute of Chemistry, University of Bialystok, K. Ciolkowskiego St. 1K, 15-245, Bialystok, Poland
  3. Laboratory of Electrochemical Power Sources, Faculty of Chemistry, University of Warsaw, Pasteur St. 1, 02-093, Warsaw, Poland
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Human Physiology
  
• 出版者：Springer New York
• ISSN：1432-1424

文摘

The objective of this research was to evaluate postmortem changes concerning electric charge of human erythrocytes and thrombocytes in fatal accidental hypothermia. The surface charge density values were determined on the basis of the electrophoretic mobility measurements of the cells conducted at various pH values of electrolyte solution. The surface charge of erythrocyte membranes after fatal accidental hypothermia increased compared to the control group within whole range of experimental pH values. Moreover, a slight shift of the isoelectric point of erythrocyte membranes towards high pH values was observed. The surface charge of thrombocyte membranes in fatal accidental hypothermia decreased at low pH compared to the control group. However, at pH range 4-, the values increased compared to the control group. The isoelectric point of thrombocyte membranes after fatal accidental hypothermia was slightly shifted towards low pH values compared to the control group. The observed changes are probably connected with the partial destruction and functional changes of the blood cell structure. Keywords Fatal accidental hypothermia Surface charge density pH measurement Erythrocyte Thrombocyte