Assessing the response of plant flavonoids to UV radiation: an overview of appropriate techniques

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• 作者：Riitta Julkunen-Tiitto (1)
  Nikolaos Nenadis (2)
  Susanne Neugart (3)
  Matthew Robson (4)
  Giovanni Agati (5)
  Jouko Veps盲l盲inen (6)
  Gaetano Zipoli (7)
  Line Nybakken (8)
  Barbro Winkler (9)
  Marcel A. K. Jansen (10)
  
  1. University of Eastern Finland ; Yliopistokatu 7 ; 80101 ; Joensuu ; Finland
  2. Aristotle University of Thessaloniki ; 541 24 ; Thessalon铆ki ; Greece
  3. Leibniz-Institute of Vegetable and Ormamental Crops Grossbeeren ; 14979 ; Grossbeeren ; Germany
  4. University of Helsinki ; 00014 ; Helsinki ; Finland
  5. CNR-Institute of Applied Physics ; 50019 ; Sesto Forentino ; Florence ; Italy
  6. University of Eastern Finland ; Yliopistonranta 1E ; 70211 ; Kuopio ; Finland
  7. CNR-Institute of Biometeorology ; Via Caproni 8 ; 50144 ; Florence ; Italy
  8. INA-Norwegian University of Life Sciences ; 1432 ; 脜s ; Norway
  9. Research Unit Environmental Simulation ; Helmholtz Zentrum M眉nchen ; 85764 ; Neuherberg ; Germany
  10. University College Cork ; Enterprise Centre ; Distillery Field ; Cork ; Ireland
  
• 关键词：Plant flavonoids ; Flavonoid extraction ; Qualitative and quantitative analyses ; Sampling and sample prehandling ; UV ; B
• 刊名：Phytochemistry Reviews
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：14
• 期：2
• 页码：273-297
• 全文大小：807 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Organic Chemistry
  Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1572-980X

文摘

Flavonoids are a large group of plant secondary metabolites that are present in most plants, and are vital for plant growth, development and protection. Among the many functions of these compounds is their contribution to stress amelioration. The accurate identification and quantification of total or individual flavonoids in plants exposed to stressful conditions (e.g. ultraviolet radiation) is challenging due to their structural diversity. The present review provides the up to date knowledge and highlights trends in plant flavonoid analysis. The review covers all steps from the field to the laboratory, focussing on UV-B effects on flavonoids, and identifying critical issues concerning sample collection, pre-treatment, extraction techniques and quantitative or qualitative analysis. A well-planned sampling and sample prehandling strategy is vital when capturing organ, tissue and developmental-stage dependent changes in flavonoids, as well as the dynamic changes due to time of UV-exposure and diurnal or seasonal parameters. A range of advanced extraction and purification techniques can facilitate the quantitative transfer of flavonoids to solvents. The advantages and disadvantages of analytical methods, including chromogenic assays, liquid and thin-layer chromatography, mass spectrometry, nuclear magnetic resonance detection, and non-destructive in situ fluorescent analysis need to be consciously evaluated in the context of the specific biological question posed. Thus, no one method can be applied to every single study of flavonoid. The message of this review is that researchers will need to carefully consider the biological process that they intend to study, and select an analytical method that optimally matches their specific objectives.