Bacterial community structure and detection of putative plant growth-promoting rhizobacteria associated with plants grown in Chilean agro-ecosystems and undisturbed ecosystems

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• 作者：Milko A. Jorquera (1)
  Nitza G. Inostroza (1)
  Lorena M. Lagos (2)
  Patricio J. Barra (2)
  Luis G. Marileo (2)
  Joaquin I. Rilling (2)
  Daniela C. Campos (1)
  David E. Crowley (3)
  Alan E. Richardson (4)
  Mar铆a L. Mora (1)
  
• 关键词：1 ; Aminocyclopropane ; 1 ; carboxylate deaminase ; Auxin ; Phytase ; Plant growth ; promoting rhizobacteria ; Rhizobacterial community
• 刊名：Biology and Fertility of Soils
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：50
• 期：7
• 页码：1141-1153
• 全文大小：1,321 KB
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• 作者单位：Milko A. Jorquera (1)
  Nitza G. Inostroza (1)
  Lorena M. Lagos (2)
  Patricio J. Barra (2)
  Luis G. Marileo (2)
  Joaquin I. Rilling (2)
  Daniela C. Campos (1)
  David E. Crowley (3)
  Alan E. Richardson (4)
  Mar铆a L. Mora (1)
  
  1. Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar 01145, Temuco, Chile
  2. Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar 01145, Temuco, Chile
  3. Department of Environmental Sciences, University of California Riverside, 900 University Ave, Riverside, CA, 92521, USA
  4. CSIRO Plant Industry, PO Box 1600, Canberra, Australia, 2601
  
• ISSN：1432-0789

文摘

Soil microorganisms with phytase- and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activities are widely studied as plant growth-promoting rhizobacteria (PGPR). Here, we explored the bacterial community structure and occurrence of putative PGPR in plants grown in agro-ecosystems and undisturbed ecosystems from northern, central, and southern Chile. Total rhizobacterial community structure was evaluated by denaturing gradient gel electrophoresis, and dominant bands present in diverse ecosystems were sequenced. Significant differences in total bacterial communities were shown with some bacterial orders (Enterobacteriales, Actinomycetales, and Rhizobiales) being highly similar to both ecosystems. Twenty-nine putative PGPR, showing phytate- and ACC-degrading activities and production of auxin, were selected from across the sites. Based on 16S rRNA gene sequencing, the putative PGPR were characterized as Enterobacteriales (Enterobacter, Serratia, Pantoea, Rahnella, Leclercia), Pseudomonas, and Bacillus, consistent with previously reported PGPR and endophytic bacteria. Beta-propeller phytase genes with similarity to Bacillus were also identified. PGPR from agro-ecosystems appeared to show higher auxin production compared to those from undisturbed ecosystems. This study demonstrates that putative PGPR are widely distributed across Chilean soils. Further understanding of their contribution to the growth and adaptation of plant hosts to local soil conditions may provide opportunity for development of new PGPR in Chilean agriculture.