Silica nanoparticles enhance autophagic activity, disturb endothelial cell homeostasis and impair angiogenesis

详细信息    查看全文

• 作者：Junchao Duan (1) (2)
  Yongbo Yu (1) (2)
  Yang Yu (1) (2)
  Yang Li (1) (2)
  Peili Huang (1) (2)
  Xianqing Zhou (1) (2)
  Shuangqing Peng (3)
  Zhiwei Sun (1) (2)
  
  1. School of Public Health ; Capital Medical University ; Beijing ; 100069 ; P.R. China
  2. Beijing Key Laboratory of Environmental Toxicology ; Capital Medical University ; Beijing ; 100069 ; P.R. China
  3. Institute of Disease Control and Prevention ; Academy of Military Medical Sciences ; Beijing ; 100071 ; P.R. China
  
• 关键词：Silica nanoparticles ; Autophagy ; Endothelium ; Angiogensis ; VEGFR2 ; Crosstalk ; Nanotoxicology
• 刊名：Particle and Fibre Toxicology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：11
• 期：1
• 全文大小：2,611 KB
• 参考文献：Report on nanotechnology: nanomaterials are widely used in commerce, but EPA faces challenges in regulating risk. Int J Occup Environ Health 16: pp. 525-539 CrossRef
  1. Li, Z, Barnes, JC, Bosoy, A, Stoddart, JF, Zink, JI (2012) Mesoporous silica nanoparticles in biomedical applications. Chem Soc Rev 41: pp. 2590-2605 CrossRef
  2. Matassoni, L, Pratesi, G, Centioli, D, Cadoni, F, Lucarelli, F, Nava, S, Malesani, P (2011) Saharan dust contribution to PM10, PM2. 5 and PM1 in urban and suburban areas of Rome: a comparison between single-particle SEM-EDS analysis and whole-sample PIXE analysis. J Environ Monit 13: pp. 732-742 CrossRef
  3. Keller, AA, McFerran, S, Lazareva, A, Suh, S (2013) Global life cycle releases of engineered nanomaterials. J Nanoparticle Res 15: pp. 1-17 CrossRef
  4. Murr, L, Bang, J, Lopez, D, Guerrero, P, Esquivel, E, Choudhuri, A, Subramanya, M, Morandi, M, Holian, A (2004) Carbon nanotubes and nanocrystals in methane combustion and the environmental implications. J Mater Sci 39: pp. 2199-2204 CrossRef
  5. Tian, L, Dai, S, Wang, J, Huang, Y, Ho, SC, Zhou, Y, Lucas, D, Koshland, CP (2008) Nanoquartz in Late Permian C1 coal and the high incidence of female lung cancer in the Pearl River Origin area: a retrospective cohort study. BMC Public Health 8: pp. 398 CrossRef
  6. Lee JE, T, Lee, N, Kim, T, Kim, J, Hyeon, T (2011) Multifunctional mesoporous silica nanocomposite nanoparticles for theranostic applications. Acc Chem Res 44: pp. 893-902 CrossRef
  7. Wang, X, Teng, Z, Wang, H, Wang, C, Liu, Y, Tang, Y, Wu, J, Sun, J, Wang, H, Wang, J, Lu, G (2014) Increasing the cytotoxicity of doxorubicin in breast cancer MCF-7 cells with multidrug resistance using a mesoporous silica nanoparticle drug delivery system. Int J Clin Exp Pathol 7: pp. 1337-1347
  Chemical Information Review Document for Silica Flour [CAS No. 14808-60-7]. National Institute of Environmental Health Sciences, National Institutes of Health, U.S. Department of Health and Human Services, Research Triangle Park, NC
  8. Organisation for Economic Co-operation and Development (OECD): / List of Manufactured Nanomaterials and List of Endpoints for Phase One of the Sponsorship Programme for the Testing of Manufactured Nanomaterials: Revision, Series on the Safety of manufactured nanomaterials No. 27-ENV/JM/MONO(2010)46. 2010. Available at: http://www.oecd.org/science/nanosafety/publicationsintheseriesonthesafetyofmanufacturednanomaterials.htm.
  9. Kumar, P, Robins, A, Vardoulakis, S, Britter, R (2010) A review of the characteristics of nanoparticles in the urban atmosphere and the prospects for developing regulatory controls. Atmos Environ 44: pp. 5035-5052 CrossRef
  10. Pope, CA, Burnett, RT, Thurston, GD, Thun, MJ, Calle, EE, Krewski, D, Godleski, JJ (2004) Cardiovascular mortality and long-term exposure to particulate air pollution: epidemiological evidence of general pathophysiological pathways of disease. Circulation 109: pp. 71-77 CrossRef
  11. Brook, RD, Rajagopalan, S, Pope, CA, Brook, JR, Bhatnagar, A, Diez-Roux, AV, Holguin, F, Hong, Y, Luepker, RV, Mittleman, MA, Peters, A, Siscovick, D, Smith, SC, Whitsel, L, Kaufman, JD (2010) Particulate matter air pollution and cardiovascular disease: an update to the scientific statement from the American Heart Association. Circulation 121: pp. 2331-2378 CrossRef
  12. Gold, DR, Mittleman, MA (2013) New insights into pollution and the cardiovascular system: 2010 to 2012. Circulation 127: pp. 1903-1913 CrossRef
  13. Organisation for Economic Co-operation and Development (OECD): / Series on the Safety of manufactured nanomaterials No. 38-ENV/JM/MONO(2013)18. 2013. Available at: http://www.oecd.org/science/nanosafety/publicationsintheseriesonthesafetyofmanufacturednanomaterials.htm.
  14. Teng, R-J, Du, J, Welak, S, Guan, T, Eis, A, Shi, Y, Konduri, GG (2012) Cross talk between NADPH oxidase and autophagy in pulmonary artery endothelial cells with intrauterine persistent pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 302: pp. L651-L663 CrossRef
  15. Stern, ST, Adiseshaiah, PP, Crist, RM (2012) Autophagy and lysosomal dysfunction as emerging mechanisms of nanomaterial toxicity. Part Fibre Toxicol 9: pp. 20 CrossRef
  16. Martinet, W, Knaapen, MW, Kockx, MM, Meyer, GR (2007) Autophagy in cardiovascular disease. Trends Mol Med 13: pp. 482-491 CrossRef
  17. Du, J, Teng, RJ, Guan, T, Eis, A, Kaul, S, Konduri, GG, Shi, Y (2012) Role of autophagy in angiogenesis in aortic endothelial cells. Am J Physiol Cell Physiol 302: pp. C383-C391 CrossRef
  18. Attar-Schneider, O, Drucker, L, Zismanov, V, Tartakover-Matalon, S, Rashid, G, Lishner, M (2012) Bevacizumab attenuates major signaling cascades and eIF4E translation initiation factor in multiple myeloma cells. Lab Invest 92: pp. 178-190 CrossRef
  19. Shen, W, Tian, C, Chen, H, Yang, Y, Zhu, D, Gao, P, Liu, J (2013) Oxidative stress mediates chemerin-induced autophagy in endothelial cells. Free Radic Biol Med 55: pp. 73-82 CrossRef
  20. Roy, A, Kolattukudy, PE (2012) Monocyte chemotactic protein-induced protein (MCPIP) promotes inflammatory angiogenesis via sequential induction of oxidative stress, endoplasmic reticulum stress and autophagy. Cell Signal 24: pp. 2123-2131 CrossRef
  21. Yu, Y, Li, Y, Wang, W, Jin, M, Du, Z, Duan, J, Sun, Z (2013) Acute toxicity of amorphous silica nanoparticles in intravenously exposed ICR mice. PLoS One 8: pp. e61346 CrossRef
  22. Yu, Y, Duan, J, Lu, Y, Li, Y, Liu, X, Zhou, X, Ho, KF, Tian, L, Sun, Z (2014) Silica nanoparticles induce autophagy and autophagic cell death in HepG2 cells triggered by reactive oxygen species. J Hazard Mater 270: pp. 176-186 CrossRef
  23. Duan, J, Yu, Y, Li, Y, Sun, Z (2013) Cardiovascular toxicity evaluation of silica nanoparticles in endothelial cells and zebrafish model. Biomaterials 34: pp. 5853-5862 CrossRef
  24. Hussain, S, Al-Nsour, F, Rice, AB, Marshburn, J, Yingling, B, Ji, Z, Zink, JI, Walker, NJ, Garantziotis, S (2012) Cerium dioxide nanoparticles induce apoptosis and autophagy in human peripheral blood monocytes. ACS Nano 6: pp. 5820-5829 CrossRef
  25. Khan, MI, Mohammad, A, Patil, G, Naqvi, SA, Chauhan, LK, Ahmad, I (2012) Induction of ROS, mitochondrial damage and autophagy in lung epithelial cancer cells by iron oxide nanoparticles. Biomaterials 33: pp. 1477-1488 CrossRef
  26. Hackenberg, S, Scherzed, A, Gohla, A, Technau, A, Froelich, K, Ginzkey, C, Koehler, C, Burghartz, M, Hagen, R, Kleinsasser, N (2014) Nanoparticle-induced photocatalytic head and neck squamous cell carcinoma cell death is associated with autophagy. Nanomedicine (Lond) 9: pp. 21-33 CrossRef
  27. Liu, HL, Zhang, YL, Yang, N, Zhang, YX, Liu, XQ, Li, CG, Zhao, Y, Wang, YG, Zhang, GG, Yang, P, Guo, F, Sun, Y, Jiang, CY (2011) A functionalized single-walled carbon nanotube-induced autophagic cell death in human lung cells through Akt-TSC2-mTOR signaling. Cell Death Dis 2: pp. e159 CrossRef
  28. Li, H, Li, Y, Jiao, J, Hu, HM (2011) Alpha-alumina nanoparticles induce efficient autophagy-dependent cross-presentation and potent antitumour response. Nat Nanotechnol 6: pp. 645-650 CrossRef
  29. Gioacchino, M, Petrarca, C, Perrone, A, Farina, M, Sabbioni, E, Hartung, T, Martino, S, Esposito, DL, Lotti, LV, Mariani-Costantini, R (2008) Autophagy as an ultrastructural marker of heavy metal toxicity in human cord blood hematopoietic stem cells. Sci Total Environ 392: pp. 50-58 CrossRef
  30. Shao, Y, Gao, Z, Feldman, T, Jiang, X (2007) Stimulation of ATG12-ATG5 conjugation by ribonucleic acid. Autophagy 3: pp. 10-16 CrossRef
  31. Kotecki, M, Zeiger, AS, Vliet, KJ, Herman, IM (2010) Calpain- and talin-dependent control of microvascular pericyte contractility and cellular stiffness. Microvasc Res 80: pp. 339-348 CrossRef
  32. Gerhardt, H, Betsholtz, C (2003) Endothelial-pericyte interactions in angiogenesis. Cell Tissue Res 314: pp. 15-23 CrossRef
  33. Wakui, S, Yokoo, K, Muto, T, Suzuki, Y, Takahashi, H, Furusato, M, Hano, H, Endou, H, Kanai, Y (2006) Localization of Ang-1, 鈭?, Tie-2, and VEGF expression at endothelial-pericyte interdigitation in rat angiogenesis. Lab Invest 86: pp. 1172-1184
  34. Tabibiazar, R, Rockson, SG (2001) Angiogenesis and the ischaemic heart. Eur Heart J 22: pp. 903-918 CrossRef
  35. Ferrara, N, Gerber, HP, LeCouter, J (2003) The biology of VEGF and its receptors. Nat Med 9: pp. 669-676 CrossRef
  36. Huang, X, Teng, X, Chen, D, Tang, F, He, J (2010) The effect of the shape of mesoporous silica nanoparticles on cellular uptake and cell function. Biomaterials 31: pp. 438-448 CrossRef
  37. Inoue, M, Ishida, T, Yasuda, T, Toh, R, Hara, T, Cangara, HM, Rikitake, Y, Taira, K, Sun, L, Kundu, RK, Quertermous, T, Hirata, K (2010) Endothelial cell-selective adhesion molecule modulates atherosclerosis through plaque angiogenesis and monocyte-endothelial interaction. Microvasc Res 80: pp. 179-187 CrossRef
  38. Sun, L, Li, Y, Liu, X, Jin, M, Zhang, L, Du, Z, Guo, C, Huang, P, Sun, Z (2011) Cytotoxicity and mitochondrial damage caused by silica nanoparticles. Toxicol In Vitro 25: pp. 1619-1629 CrossRef
  39. Guharoy, M, Szabo, B, Contreras Martos, S, Kosol, S, Tompa, P (2013) Intrinsic structural disorder in cytoskeletal proteins. Cytoskeleton (Hoboken) 70: pp. 550-571 CrossRef
  40. Ehrenberg, M, McGrath, JL (2005) Binding between particles and proteins in extracts: implications for microrheology and toxicity. Acta Biomater 1: pp. 305-315 CrossRef
  41. Lee, JY, Koga, H, Kawaguchi, Y, Tang, W, Wong, E, Gao, YS, Pandey, UB, Kaushik, S, Tresse, E, Lu, J, Taylor, JP, Cuervo, AM, Yao, TP (2010) HDAC6 controls autophagosome maturation essential for ubiquitin-selective quality-control autophagy. EMBO J 29: pp. 969-980 CrossRef
  42. Mostowy, S, Cossart, P (2011) Autophagy and the cytoskeleton: new links revealed by intracellular pathogens. Autophagy 7: pp. 780-782 CrossRef
  43. Duan, J, Yu, Y, Li, Y, Zhou, X, Huang, P, Sun, Z (2013) Toxic effect of silica nanoparticles on endothelial cells through DNA damage response via Chk1-dependent G2/M checkpoint. PLoS One 8: pp. e62087 CrossRef
  44. Dromparis, P, Michelakis, ED (2013) Mitochondria in vascular health and disease. Annu Rev Physiol 75: pp. 95-126 CrossRef
  45. Green, DR, Galluzzi, L, Kroemer, G (2011) Mitochondria and the autophagy-inflammation-cell death axis in organismal aging. Science 333: pp. 1109-1112 CrossRef
  46. Mizushima, N, Yoshimori, T (2007) How to interpret LC3 immunoblotting. Autophagy 3: pp. 542-545 CrossRef
  47. Ungvari, Z, Labinskyy, N, Gupte, S, Chander, PN, Edwards, JG, Csiszar, A (2008) Dysregulation of mitochondrial biogenesis in vascular endothelial and smooth muscle cells of aged rats. Am J Physiol Heart Circ Physiol 294: pp. H2121-H2128 CrossRef
  48. Dudek, SM, Garcia, JG (2001) Cytoskeletal regulation of pulmonary vascular permeability. J Appl Physiol (1985) 91: pp. 1487-1500
  49. VandenBerg, E, Reid, MD, Edwards, JD, Davis, HW (2004) The role of the cytoskeleton in cellular adhesion molecule expression in tumor necrosis factor-stimulated endothelial cells. J Cell Biochem 91: pp. 926-937 CrossRef
  50. He, C, Klionsky, DJ (2009) Regulation mechanisms and signaling pathways of autophagy. Annu Rev Genet 43: pp. 67-93 CrossRef
  51. Pyo, JO, Nah, J, Jung, YK (2012) Molecules and their functions in autophagy. Exp Mol Med 44: pp. 73-80 CrossRef
  52. Cho, CH, Lee, CS, Chang, M, Jang, IH, Kim, SJ, Hwang, I, Ryu, SH, Lee, CO, Koh, GY (2004) Localization of VEGFR-2 and PLD2 in endothelial caveolae is involved in VEGF-induced phosphorylation of MEK and ERK. Am J Physiol Heart Circ Physiol 286: pp. H1881-H1888 CrossRef
  53. Duan, J, Yu, Y, Shi, H, Tian, L, Guo, C, Huang, P, Zhou, X, Peng, S, Sun, Z (2013) Toxic effects of silica nanoparticles on zebrafish embryos and larvae. PLoS One 8: pp. e74606 CrossRef
  54. Xu, J, Sun, L, Li, J, Liang, J, Zhang, H, Yang, W (2011) FITC and Ru(phen)32+ co-doped silica particles as visualized ratiometric pH indicator. Nanoscale Res Lett 6: pp. 561 CrossRef
  
• 刊物主题：Pharmacology/Toxicology; Pneumology/Respiratory System; Nanotechnology and Microengineering;
• 出版者：BioMed Central
• ISSN：1743-8977

文摘

Background Given that the effects of ultrafine fractions ( Methods and results Ultrastructural changes of autophagy were observed in both vascular endothelial cells and pericytes in the heart of ICR mice by TEM. Autophagic activity and impaired angiogenesis were further confirmed by the immunohistochemistry staining of LC3 and VEGFR2. In addition, the immunohistochemistry results showed that SiNPs had an inhibitory effect on ICAM-1 and VCAM-1, but no obvious effect on E-selectin in vivo. The disruption of F-actin cytoskeleton occurred as an initial event in SiNPs-treated endothelial cells. The depolarized mitochondria, autophagic vacuole accumulation, LC3-I/LC3-II conversion, and the down-regulation of cellular adhesion molecule expression were all involved in the disruption of endothelial cell homeostasis in vitro. Western blot analysis indicated that the VEGFR2/PI3K/Akt/mTOR and VEGFR2/MAPK/Erk1/2/mTOR signaling pathway was involved in the cardiovascular toxicity triggered by SiNPs. Moreover, there was a crosstalk between the VEGFR2-mediated autophagy signaling and angiogenesis signaling pathways. Conclusions In summary, the results demonstrate that SiNPs induce autophagic activity in endothelial cells and pericytes, subsequently disturb the endothelial cell homeostasis and impair angiogenesis. The VEGFR2-mediated autophagy pathway may play a critical role in maintaining endothelium and vascular homeostasis. Our findings may provide experimental evidence and explanation for cardiovascular diseases triggered by nano-sized particles.