The insulin-like receptor gene expression in the tissues synthesizing gonadotropic hormones at sexual maturation of Drosophila melanogaster females

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• 作者：O. V. Andreenkova ; N. V. Adonyeva ; M. A. Eremina…
• 关键词：juvenile hormone ; 20 ; hydroxyecdysone ; insulin signaling pathway ; Drosophila
• 刊名：Russian Journal of Genetics
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：52
• 期：11
• 页码：1214-1217
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics;
• 出版者：Pleiades Publishing
• ISSN：1608-3369
• 卷排序：52

文摘

The insulin/insulin-like growth factor signaling pathway is involved in the regulation of the synthesis of insect gonadotropic hormones, juvenile (JH) and 20-hydroxyecdysone (20E). We carried out the immunohistochemical analysis of the insulin receptor (InR) expression in the corpus allatum (the JH-producing gland) and in the ovarian follicular cells (a site for the synthesis of 20E precursor, ecdysone) in the process of sexual maturation of D. melanogaster females and examined the influence of exogenous JH on the InR expression in these tissues. For the first time, it was demonstrated that InR was expressed in follicular cells and that its expression in corpus allatum and follicular cells of Drosophila females was stage-specific, i.e., the expression intensity in young females greatly exceeded that in mature individuals. We also found a negative feedback loop in the regulation of JH levels by the insulin signaling pathway in Drosophila adults: the experimental increase in the JH titers in young females dramatically reduced the InR expression intensity in corpus allatum and follicular cells.