Differential expression of ATP-gated P2X receptors in DRG between chronic neuropathic pain and visceralgia rat models

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• 作者：Lin Chen ; Yu-wei Liu ; Kai Yue ; Qin Ru ; Qi Xiong ; Bao-miao Ma…
• 关键词：Neuropathic pain ; Visceralgia ; DRG ; P2X ; Rs ; Expression characteristics
• 刊名：Purinergic Signalling
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：12
• 期：1
• 页码：79-87
• 全文大小：4,123 KB
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• 作者单位：Lin Chen (1)
  Yu-wei Liu (2)
  Kai Yue (1)
  Qin Ru (1)
  Qi Xiong (1)
  Bao-miao Ma (1)
  Xiang Tian (1)
  Chao-ying Li (1)
  
  1. Wuhan Institutes of Biomedical Sciences, Jianghan University, Wuhan, 430056, China
  2. School of Medicine, Jianghan University, Wuhan, 430056, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Pharmacology and Toxicology
  Human Physiology
  Neurosciences
  Cancer Research
  
• 出版者：Springer Netherlands
• ISSN：1573-9546

文摘

There are divergences between neuropathic pain and visceralgia in terms of the duration, location, and character of hyperalgesia. It is generally recognized that nociceptive receptors, including P2X receptors, may play different roles in nociceptive mechanisms. The different roles of P2X1–7 receptors have not been fully understood both in neuropathic pain and visceral hyperalgesia. In order to explore the different expressions of P2X1–7 receptors in these two hyperalgesia models, the lumbosacral dorsal root ganglion (DRG) neurons from rat sciatic nerve chronic constriction injury (CCI) model and neonatal colorectal distention (NCRD) model were studied (both the primary nociceptive neuron afferents of those two models projected to the same segment of spinal cord). Both immunohistochemistry (IHC) technique and real-time fluorescence quantitative polymerase chain reaction (RT-PCR) technology were applied to analyze the protein expression levels and nucleic acid of P2X1–7 receptors. We found that except P2X2 and P2X3, the expression levels of P2X1 and P2X5 receptors increased in neuropathic pain while those expression levels of P2X4, P2X6, and P2X7 receptors increased in visceral pain. Our results also suggested that in addition to P2X2/3 heteromeric, other P2X subunits may also involved in generation heteromeric such as P2X1/5 and/or P2X2/5 in neuropathic pain and P2X4/6 and/or P2X4/7 in visceral pain. Keywords Neuropathic pain Visceralgia DRG P2X-Rs Expression characteristics