Enhancement of cadmium tolerance and accumulation by introducing Perilla frutescens (L.) Britt var. frutescens genes in Nicotiana tabacum L. plants
详细信息 查看全文
- 作者：Keqiang Wei ; Shengxi Pang ; Junxian Yang…
- 关键词：Cadmium ; Hybrid breeding ; Perilla frutescens ; Phytoremediation ; Nicotiana tabacum
- 刊名：Environmental Science and Pollution Research
- 出版年：2015
- 出版时间：April 2015
- 年：2015
- 卷：22
- 期：7
- 页码：5405-5416
- 全文大小：2,194 KB
- 参考文献：1. Andrianov, V, Borisjuk, N, Pogrebnyak, N, Brinker, A, Dixon, J, Spitsin, S, Flynn, J, Matyszczuk, P, Andryszak, K, Laurelli, M, Golovkin, M, Koprowski, H (2010) Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass. Plant Biotechnol J 3: pp. 277-287 CrossRef
2. Assun??o, AG, Pieper, B, Vromans, J, Lindhout, P, Aarts, MG, Schat, H (2006) Construction of a genetic linkage map of Thlaspi caerulescens and quantitative trait loci analysis of zinc accumulation. New Phytol 1: pp. 21-32 CrossRef
3. Barceló, J, Poschenrieder, C (2003) Phytoremediation: principles and perspectives. Contrib Sci 3: pp. 333-334
4. Bhargava, A, Carmona, FF, Bhargava, M, Srivastava, S (2012) Approaches for enhanced phytoextraction of heavy metals. J Environ Manage 105: pp. 103-120 CrossRef
5. Carolina, TM, Alesso, M, Acosta, M, Olsina, R, Fernández, LP (2013) Determination of cadmium in tobacco by solid surface fluorescence using nylon membranes coated with carbon nanotubes. Talanta 107: pp. 61-66 CrossRef
6. Chen, GG, Zhang, JY, Guo, YL (2004) Analysis of volatile components of fresh Perilla frutescens (L.) Britt. var. acuta (Thunb.) Kudo by headspace GC/MS. J Essent Oil Res 5: pp. 435-436 CrossRef
7. Deniau, AX, Pieper, B, Bookum, WM, Lindhout, P, Aarts, MG, Schat, H (2006) QTL analysis of cadmium and zinc accumulation in the heavy metal hyperaccumulator Thlaspi caerulescens. Theor Appl Genet 113: pp. 907-920 CrossRef
8. Douchiche, O, Chaibi, W, Morvan, C (2012) Cadmium tolerance and accumulation characteristics of mature flax, cv. Hermes: Contribution of the basal stem compared to the root. J Hazard Mater 235-36: pp. 101-107 CrossRef
9. Dushenkov, S, Skarzhinskaya, M, Glimelius, K, Gleba, D, Raskin, I (2002) Bioengineering of a phytoremediation plant by means of somatic hybridization. Int J Phytoremed 4: pp. 117-126 CrossRef
10. Gorinova, N, Nedkovska, M, Todorovska, E, Simova-Stoilova, L, Stoyanova, Z, Georgieva, K, Demirevska-Kepova, K, Atanassov, A, Herzig, R (2007) Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity. Environ Pollut 145: pp. 161-170 CrossRef
11. Grant, CA, Clarke, JM, Duguid, S, Chaney, RL (2008) Selection and breeding of plant cultivars to minimize cadmium accumulation. Sci Total Environ 2-: pp. 301-310 CrossRef
12. Grispen, VMJ, Hakvoort, HWJ, Bliek, T, Verkleij, JAC, Schat, H (2011) Combined expression of the Arabidopsis metallothionein MT2b and the heavy metal transporting ATPase HMA4 enhances cadmium tolerance and the root to shoot translocation of cadmium and zinc in tobacco. Environ Exp Bot 72: pp. 71-76 CrossRef
13. Hertstein, U, Jager, HJ (1986) Tolerances of different populations of three grass species to cadmium and other metals. Environ Exp Bot 26: pp. 309-319 CrossRef
14. Kang, XW, Wei, ZZ (1986) Cross breeding of tobacco. Shanxi Science and Education Publishing House, Taiyuan
15. Khoudi, H, Maatar, Y, Gouiaa, S, Masmoudi, K (2012) Transgenic tobacco plants expressing ectopically wheat H+-pyrophosphatase (H+-PPase) gene TaVP1 show enhanced accumulation and tolerance to cadmium. J Plant Physiol 169: pp. 98-103
文摘
The tobacco has the genetic potential to remove toxic metals from the soil. To develop hyperaccumulating tobacco plants, distant hybridization between tobacco (Nicotiana tabacum L.), a high-biomass crop, and Perilla frutescens (L.) Britt var. frutescens, a newfound Cd-hyperaccumulator species, was carried out using a novel method viz. pollination following grafting. Their hybrid nature was preliminarily confirmed by phenotype, isozyme pattern, random amplified polymorphic DNA (RAPD) and metabolites analysis. About 120 putative F2 hybrids derived from the cross-combination [(N. sylvestris Speg. & Comes rootstock--em class="a-plus-plus">N. tabacum L. var. 78-4 scion) × P. frutescens (L.) Britt var. frutescens] were then subjected to up to 300?μM CdCl2 in hydroponic conditions for 10?days. Results showed five seedlings were more resistant to Cd than female parent and accumulated 314.6?±-9.9?mg?kg? Cd in their aerial biomass, which was 5.7 times greater than that in "78-04" tobacco (47.2?±-.56?mg?kg?) (P?≤-.05). Two of these seedlings exceeded male parent P. frutescens in the Cd concentration of shoots and reached 424 and 396?mg?kg?, which was 13?% and 6?% greater for that of perilla (374.2?±-0.38?mg?kg?), respectively. Compared with parents, two other F2 hybrids tended to accumulate more Cd in the root with bioconcentration factor (BCF) 7.05 and 5.17, respectively. Only one hybrid showed lower Cd concentration but transferred Cd more effectively from the root to the shoot than parents and other F2 hybrids, with the maximum translocation factor (TF) value 1.37. These indicated that the introduction of P. frutescens genes could obviously enhance the cadmium tolerance and accumulation of superior individuals.