Screening, Isolation and Identification of Probiotic Producing Lactobacillus acidophilus Strains EMBS081 & EMBS082 by 16S rRNA Gene Sequencing

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• 作者：Harshpreet Chandok ; Pratik Shah…
• 关键词：Lactobacillus acidophilus ; Probiotic Lactobacillus ; 16srRNA sequencing ; Lactobacillus acidophilus strain EMBS081 ; Lactobacillus acidophilus strain EMBS082
• 刊名：Interdisciplinary Sciences: Computational Life Sciences
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：7
• 期：3
• 页码：242-248
• 全文大小：841 KB
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• 作者单位：Harshpreet Chandok (1)
  Pratik Shah (1)
  Uday Raj Akare (1)
  Maliram Hindala (1)
  Sneha Singh Bhadoriya (1)
  G. V. Ravi (1)
  Varsha Sharma (1)
  Srinivas Bandaru (2)
  Pragya Rathore (3)
  Anuraj Nayarisseri (1)
  
  1. Bioinformatics Research Laboratory, Eminent Biosciences, Indore, 452 010, India
  2. Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500 016, India
  3. Department of Biotechnology, Sanghvi Institute of Management & Science, Indore, 453 331, India
  
• 刊物主题：Computer Appl. in Life Sciences; Computational Biology/Bioinformatics; Statistics for Life Sciences, Medicine, Health Sciences; Theoretical and Computational Chemistry; Theoretical, Mathematical and Computational Physics; Computational Science and Engineering;
• 出版者：International Association of Scientists in the Interdisciplinary Areas
• ISSN：1867-1462

文摘

16S rDNA sequencing which has gained wide popularity amongst microbiologists for the molecular characterization and identification of newly discovered isolates provides accurate identification of isolates down to the level of sub-species (strain). Its most important advantage over the traditional biochemical characterization methods is that it can provide an accurate identification of strains with atypical phenotypic characters as well. The following work is an application of 16S rRNA gene sequencing approach to identify a novel species of Probiotic Lactobacillus acidophilus. The sample was collected from pond water samples of rural and urban areas of Krishna district, Vijayawada, Andhra Pradesh, India. Subsequently, the sample was serially diluted and the aliquots were incubated for a suitable time period following which the suspected colony was subjected to 16S rDNA sequencing. The sequence aligned against other species was concluded to be a novel, Probiotic L. acidophilus bacteria, further which were named L. acidophilus strain EMBS081 & EMBS082. After the sequence characterization, the isolate was deposited in GenBank Database, maintained by the National Centre for Biotechnology Information NCBI. The sequence can also be retrieve from EMBL and DDBJ repositories with accession numbers JX255677 and KC150145. Keywords Lactobacillus acidophilus Probiotic Lactobacillus 16srRNA sequencing Lactobacillus acidophilus strain EMBS081 Lactobacillus acidophilus strain EMBS082