In vitro evaluation of anticancer properties of exopolysaccharides from Lactobacillus acidophilus in colon cancer cell lines

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• 作者：Venkataraman Deepak ; Sharavan Ramachandran…
• 关键词：L. acidophilus ; Exopolysaccharides ; Colon cancer ; RT ; qPCR ; Antioxidative
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：52
• 期：2
• 页码：163-173
• 全文大小：1,895 KB
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• 作者单位：Venkataraman Deepak (1) (2)
  Sharavan Ramachandran (2)
  Reham Mohammed Balahmar (2)
  Sureshbabu Ram Kumar Pandian (1)
  Shiva D. Sivasubramaniam (2)
  Hariharan Nellaiah (1)
  Krishnan Sundar (1)
  
  1. Department of Biotechnology, Kalasalingam University, Krishnankoil, 626126, Tamil Nadu, India
  2. School of Science and Technology, Nottingham Trent University, Nottingham, NG11 8NS, UK
  
• 刊物主题：Cell Biology; Developmental Biology; Stem Cells; Cell Culture; Animal Genetics and Genomics;
• 出版者：Springer US
• ISSN：1543-706X

文摘

The present work aims at studying the effect of exopolysaccharides (EPS) from Lactobacillus acidophilus on the colon cancer cell lines in vitro. Initial analysis showed that EPS has antioxidative properties. EPS was also found to induce cytotoxicity in two colon cancer cell lines, viz. HCT15 and CaCo2 under normoxia and hypoxia. The membrane integrity was also found to be affected in EPS-treated cells. Once the toxic concentration was determined (5 mg/ml), the effect of EPS on the messenger RNA (mRNA) expression of various genes was studied by quantitative real-time (RT)-PCR under both normoxic and hypoxic conditions. The results suggest that EPS downregulated the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α (HIF-1α) and upregulated the expression of tissue inhibitor of metalloproteinases-3 (TIMP-3), hypoxia-inducible factor-2α (HIF-2α), and hemeoxygenase-1 (HO-1). An increase in plasminogen activator inhibitor-1 (PAI-1) was also observed. These results show that EPS may inhibit the expressions of genes involved in tumor angiogenesis and survival. Increase in the expression of HO-1 also shows that EPS have antioxidative properties. Keywords L. acidophilus Exopolysaccharides Colon cancer RT-qPCR Antioxidative