Isolation of levoglucosan-assimilating microorganisms from soil and an investigation of their levoglucosan kinases

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• 作者：Huijun Xie ; Xuliang Zhuang ; Zhihui Bai ; Hongyan Qi and Hongxun Zhang
• 关键词：1 ; 6 ; Anhydro ; β ; d ; glucose ; levoglucosan kinase ; levoglucosan ; assimilating organisms ; protein property ; protein purification ; soil
• 刊名：World Journal of Microbiology and Biotechnology
• 出版年：2006
• 出版时间：September, 2006
• 年：2006
• 卷：22
• 期：9
• 页码：887-892
• 全文大小：263 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

The phosphorylation activities of glucose and levoglucosan (1,6-anhydro-β-d-glucopyranose, LG) were studied in 26 types of LG-assimilating microorganisms isolated from four types of soil in China. The results showed that activities of LG kinase production in most of the yeasts were lower than those of filamentous fungi, and that the highest filamentous fungal activities (0.61 U/mg protein) was approximately twice that of yeast. The filamentous fungi and yeast with highest LG kinase production activity were identified by DNA sequence analysis of 18S or 26S rRNA gene fragments respectively. The identities of them were Alternaria alternata, Eupenicillium javanicum, Aspergillus niger, Penicillium herquei, Cryptococcus laurentii, Cryptococcus flavescens, Cryptococcus luteolus and Rhodotorula aurantiaca, respectively. In addition, the LG kinases from both types of organisms were purified. The effects of temperature and pH on LG kinase activity were also studied. There was very little change in activity between pH 7 to 10 and at temperatures below 30 °C. The apparent K _m values for ATP were also similar in different microorganisms, but K _m values for LG ranged from 48 to 102 mM.