Screening and Characterization of the High-Cellulase-Producing Strain Aspergillus glaucus XC9
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- 作者：Chang Xu (1)
Minnan Long (1)
Xiaobing Wu (1)
Huijuan Xu (1)
Zhongan Chen (2)
Fengzhang Zhang (1)
Liangshu Xu (1) - 关键词：Aspergillus glaucus ; cellulase activity ; saccharification
- 刊名：Frontiers in Biology
- 出版年：2006
- 出版时间：January 2006
- 年：2006
- 卷：1
- 期：1
- 页码：35-40
- 全文大小：160KB
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Minnan Long (1)
Xiaobing Wu (1)
Huijuan Xu (1)
Zhongan Chen (2)
Fengzhang Zhang (1)
Liangshu Xu (1)
1. Institute of Microbiology and Marine Medicine, School of Life Sciences, Xiamen University, Xiamen, 361005, China
2. Haicang Waste Water Treatment Plant, Xiamen, 361026, China - ISSN：1674-7992
文摘
Cellulose is a kind of renewable resource that is abundant in nature. It can be degraded by microorganisms such as mildew. A mildew strain with high cellulase activity was isolated from mildewy maize cob and classified as Aspergillus glaucus XC9 by morphological and 18S rRNA gene sequence analyses. We studied the effects of nitrogen source, initial pH, temperature, incubation time, medium composition, and surfactants on cellulase production. Maximal activities of carboxymethylcellulase (6,812 U/g dry koji) and filter paperase (172 U/g dry koji) were obtained in conditions as follows: initial pH, 5.5-.0; temperature, 30°C; cultivation period, 3- days; inoculum ratio, 6% (vol/vol); sugarcane bagasse/wheat bran ratio, 4:6. When bagasse was used as substrate and mixed with wet koji at a 1:1 (wt/wt) ratio, the yield of reducing sugars was 36.4%. The corresponding conversion rate of cellulose to reducing sugars went as high as 81.9%. The results suggest that A. glaucus XC9 is a preferred candidate for cellulase production.