Iridoid and phenylethanoid glycoside production and phenotypical changes in plants regenerated from hairy roots of Rehmannia glutinosa Libosch.

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• 作者：Ewelina Pi?tczak ; ?ukasz Ku?ma ; Ewa Ska?a…
• 关键词：Agrobacterium rhizogenes ; Iridoid glycosides ; Phenylethanoid glycosides ; Rehmannia glutinosa ; Transformed plants
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：122
• 期：2
• 页码：259-266
• 全文大小：938 KB
• 参考文献：Angiosperm Phylogeny Group (2009) An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Bot J Linn Soc 161:105-21View Article
  Bercetche J, Chriqui D, Adam S, David C (1987) Morphogenetic and cellular reorientation induced by Agrobacterium rhizogenes (strains 1855, 2659 and 8196) on carrot, pea and tobacco. Plant Sci 52:195-10View Article
  Casanova E, Zuker A, Trillas MI, Moysset L, Vainstein A (2003) The rolC gene in carnation exhibits cytokinin and auxin-like activities. Sci Hort 97:321-33View Article
  Chaudhuri KN, Ghosh B, Tepfer D, Jha S (2006) Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation. Plant Cell Rep 25:1059-066PubMed View Article
  Choi PS, Kim YD, Choi KM, Chung HJ, Choi DW, Liu JR (2004) Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus. Plant Cell Rep 22:828-31. doi:10.-007/?s00299-004-0765-3 PubMed View Article
  Christey M (2001) Use of Ri-mediated transformation for production of transgenic plants. In Vitro Cell Dev Biol Plant 37:687-00. doi:10.-079/?IVP2001203 View Article
  Gangopadhyay M, Chakraborty D, Bhattacharyya S, Bhattacharya S (2010) Regeneration of transformed plants from hairy roots of Plumbago indica. Plant Cell Tiss Organ Cult 102:109-14. doi:10.-007/?s11240-010-9702-z View Article
  Giri A, Narasu ML (2000) Transgenic hairy roots: recent trends and applications. Biotechnol Adv 18:1-2PubMed View Article
  Grishchenko OV, Kiselev KV, Tchernoded GK, Fedoreyev SA, Veselova MV, Bulgakov VP, Zhuravlev YN (2013) The influence of the rolC gene on isoflavonoid production in callus cultures of Maackia amurensis. Plant Cell Tiss Organ Cult 113:429-35. doi:10.-007/?s11240-012-0283-x View Article
  Hwang SJ (2005) Growth characteristics and catalpol production in chinese foxglove (Rehmannia glutinosa Liboschitz) hairy roots transformed with Agrobacterium rhizogenes ATCC15834. J Plant Biol 48:380-86 (in Korean)View Article
  Kamble S, Gopalakrishnan R, Eapen S (2011) Production of camptothecin by hairy roots and regenerated transformed shoots of Ophiorrhiza rugosa var. decumbens. Nat Prod Res 25:1762-765. doi:10.-080/-4786419.-011.-59469 PubMed View Article
  Kang HJ, Anbazhagan VR, You XL, Moon HK, Yi JS, Choi YE (2006) Production of transgenic Aralia elata regenerated from Agrobacterium rhizogenes-mediated transformed roots. Plant Cell Tiss Org Cult 85:187-96. doi:10.-007/?s11240-005-9070-2 View Article
  Kitagawa I, Fukuda Y, Taniyama T, Yoshikawa M (1991) Chemical studies on crude drug processing. VII. On the constituents of Rehmanniae radix. (1): absolute stereostructures of rehmaglutins A, B and D isolated from Chinese Rehmanniae Radix, the dried root of Rehmannia glutinosa Libosch. Chem Pharm Bull 39:1171-176View Article
  Mehrotra S, Goel MK, Rahman LU, Kukreja AK (2013) Molecular and chemical characterization of plants regenerated from Ri-mediated hairy root cultures of Rauwolfia serpentina. Plant Cell Tiss Organ Cult 114:31-8. doi:10.-007/?s11240-013-0302-6 View Article
  Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco culture. Physiol Plant 15:473-97
  Palazon J, Cusido RM, Roig C, Pinol MT (1998) Expression of the rolC gene and nicotine production in transgenic roots and their regenerated plants. Plant Cell Rep 17:384-90View Article
  Pi?tczak E, Królicka A, Wysokińska H (2006) Genetic transformation of Centaurium erythraea Rafn by Agrobacterium rhizogenes and the production of secoiridoids. Plant Cell Rep 25:1308-315. doi:10.-007/?s00299-006-0155-0
  Pi?tczak E, Królicka A, Wielanek M, Wysokińska H (2012) Hairy root cultures of Rehmannia glutinosa and production of iridoid and phenylethanoid glycosides. Acta Physiol Plant 34:2215-224. doi:10.-007/?s11738-012-1022-y View Article
  Pi?tczak E, Grzegorczyk-Karolak I, Wysokińska H (2014) Micropropagation of Rehmannia glutinosa Libosch.: production of phenolics and flavonoids and evaluation of antioxidant activity. Acta Physiol Plant 36:1693-702. doi:10.-007/?s11738-014-1544-6 View Article
  Pi?tczak E, Ku?ma ?, Sitarek P, Wysokińska H (2015) Shoot organogenesis, molecular analysis and secondary metabolite production of micropropagated Rehmannia glutinosa Libosch. Plant Cell Tiss Org Cult?120:539-49. doi:10.-007/?s11240-014-0620-3
  Zhang RX, Li MX, Jia ZP (2008) Rehmannia glutinosa: review of botany, chemistry and pharmacology. J Ethnopharmacol 117:199-14. doi:10.-016/?j.?jep.-008.-2.-18 PubMed View Article
  Zhou YQ, Duan HY, Zhou CE, Li JJ, Gu FP, Wang F, Hang ZY, Gao ZM (2009) Hairy root induction and plant regeneration of Rehmannia glutinosa Libosch. f. hueichingensis Hsiao via Agrobacterium rhizogenes-mediated transformation. Russ J Plant P
• 作者单位：Ewelina Pi?tczak (1)
  ?ukasz Ku?ma (1)
  Ewa Ska?a (1)
  Marta ?ebrowska (2)
  Ewa Balcerczak (2)
  Halina Wysokińska (1)
  
  1. Department of Biology and Pharmaceutical Botany, Medical University of ?ód?, Muszyńskiego 1, 90-151, Lodz, Poland
  2. Laboratory of Molecular Diagnostic and Pharmacogenomics, Department of Pharmaceutical Biochemistry and Molecular Diagnostic, Medical University of ?ód?, Muszyńskiego 1, 90-151, Lodz, Poland
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Spontaneous shoot regeneration was obtained from the hairy roots of Rehmannia glutinosa cultured in liquid woody plant medium for 4?weeks in darkness after induction by Agrobacterium rhizogenes strain A4. These shoots were proliferated on solid Murashige and Skoog (MS) medium in 300?mL glass jars and in liquid MS medium in a 5?L nutrient sprinkle bioreactor. These media were supplemented with 0.1?mg?L? of indole-3-acetic acid (IAA) and 1.0?mg?L? of 6-benzylaminopurine. All shoots became rooted on MS agar medium containing IAA or indole-3-butyric acid within 4?weeks. The plantlets were then transferred into soil and grew in a greenhouse. The transgenic nature of the plants was confirmed by polymerase chain reaction (PCR) and reverse-transcriptase PCR analyses. The pRi-regenerated transformants demonstrated a few phenotypical alterations. Production of six iridoid (catalpol, aucubin, loganin, catalposide, harpagide, harpagoside) and two phenylethanoid (verbascoside and isoverbascoside) glycosides was determined in shoots cultured in vitro and leaves and roots of 6- and 12-month-old plants by ultra high performance liquid chromatography. The shoot culture was able to synthesize low amounts of catalpol, verbascoside and isoverbascoside. Leaves and roots of pRi-regenerants accumulated similar (harpagoside, isoverbascoside), higher (catalposide, aucubin, harpagide, verbascoside) or lower (catalpol, loganin) levels of the metabolites in comparison with untransformed R. glutinosa plants and their content varied with the age of the plants and the organ analyzed. However, the increase in shoot and root biomass of transformed plants resulted in a significantly greater yield of all analyzed compounds.