Molecular characterization of SjBIRP, another apoptosis inhibitor, from Schistosoma japonicum
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- 作者：Jinwei Dao (1) (2)
Lihui Zhu (1)
Rong Luo (1)
Chao Hu (1)
Yuqing Wang (1)
Hao Li (1)
Ke Lu (1)
Jinming Liu (1)
Jiaojiao Lin (1)
Guofeng Cheng (1) (2) - 关键词：Apoptosis ; Inhibitor of apoptosis protein ; Schistosoma japonicum ; Development ; Drug target
- 刊名：Parasitology Research
- 出版年：2014
- 出版时间：November 2014
- 年：2014
- 卷：113
- 期：11
- 页码：4065-4071
- 全文大小：1,092 KB
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Lihui Zhu (1)
Rong Luo (1)
Chao Hu (1)
Yuqing Wang (1)
Hao Li (1)
Ke Lu (1)
Jinming Liu (1)
Jiaojiao Lin (1)
Guofeng Cheng (1) (2)
1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, 200241, Shanghai, China
2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yang zhou, 225009, China- ISSN：1432-1955
- 作者单位：Jinwei Dao (1) (2)
文摘
Inhibitor of apoptosis proteins (IAP) play an important role in the regulation of apoptotic processes and are defined by the presence of baculoviral IAP repeat (BIR) domains. Here, we characterized a cDNA fragment (SjBIRP) synthesized from the RNA of Schistosoma japonicum, which was found to contain the BIR domain, suggesting that it could encode a potential IAP. Real-time PCR analyses indicated that SjBIRP transcription was detected at several stages of the schistosome’s lifecycle, with increased levels present in schistosomula (7?days). In addition, the SjBIRP was highly expressed in adult females as compared to adult males. A functional assay showed that SjBIRP could inhibit caspase3/7 activity in both HeLa cells and schistosome lysates. Furthermore, SjBIRP expression profiles varied between different hosts of S. japonicum. Taken together, our preliminary studies suggest that SjBIRP may play a functional role in the regulation of apoptosis in schistosomes, and that it could be a potential drug target for schistosomiasis control.
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