Isolation and characterization of twenty-eight polymorphic microsatellite markers in Odontobutis potamophila and cross-amplification in other Gobioidei

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• 作者：Fei Zhu (1) (2) (3)
  Jian Luo (2)
  Shaowu Yin (1) (3)
  Lijuan Zhang (1) (3)
  Xinyuan Hou (1) (3)
  Yali Hu (1) (3)
  
• 关键词：Odontobutis potamophila ; Microsatellite markers ; Cross ; amplification
• 刊名：Conservation Genetics Resources
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：6
• 期：3
• 页码：601-604
• 全文大小：163 KB
• 参考文献：1. Excoffier L, Lischer H (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564-67 CrossRef
  2. Huckstorf V (2012) / Odontobutis potamophilus. In: IUCN 2012. IUCN red list of threatened species
  3. Lalitha S (2000) Primer Premier 5. Biotechnol Softw Internet Rep 6:270-72 CrossRef
  4. Oosterhout CV, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535-38 CrossRef
  5. Sun XW, Lu CY, Liang LQ (2005) Isolation of micro-satellite with enrichment by magnetic beads grass carp, / Ctenopharyngodon idella. J Fish China 29:482-86
  
• 作者单位：Fei Zhu (1) (2) (3)
  Jian Luo (2)
  Shaowu Yin (1) (3)
  Lijuan Zhang (1) (3)
  Xinyuan Hou (1) (3)
  Yali Hu (1) (3)
  
  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China
  2. College of Ocean, Hainan University, Haikou, 570228, China
  3. Co-Innovation Center for Marine Bio-Industry Technology, Lianyungang, 222005, China
  
• ISSN：1877-7260

文摘

Odontobutis potamophila is widely distributed in river systems of southeast China. Twenty-eight microsatellite loci were isolated and characterized for this species. Number of alleles ranged from 2 to 19 per locus, and the observed and expected heterozygosity were 0.161-.967 and 0.207-.928, respectively. No evidence of linkage disequilibrium was detected. The significant departures from Hardy–Weinberg expectations at three loci suggested the presence of null alleles. These microsatellite loci will be useful for studying population genetic variation. Cross-amplification of these microsatellite loci indicated that many are polymorphic in four closely related species of Gobioidei.