Determination of Tyramine in Hair Samples by GC–MS

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• 作者：Lina Gao ; Huiya Yuan ; Juan Zhu ; Kenji Hara ; Junting Liu
• 关键词：Gas chromatography–mass spectrometry ; Tyramine ; Hair ; Heptafluorobutyryl chloride ; Schizophrenia
• 刊名：Chromatographia
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：79
• 期：1-2
• 页码：103-108
• 全文大小：511 KB
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• 作者单位：Lina Gao (1)
  Huiya Yuan (1)
  Juan Zhu (2)
  Kenji Hara (3)
  Junting Liu (1)
  
  1. School of Forensic Medicine, China Medical University, Shenyang, 110013, People’s Republic of China
  2. School of Pharmacy, China Medical University, Shenyang, 110013, People’s Republic of China
  3. Department of Forensic Medicine, Fukuoka University School of Medicine, Fukuoka, 814-0180, Japan
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

A simple and sensitive method for the determination of tyramine in hair as N-heptafluorobutyryl derivative by gas chromatography–mass spectrometry has been developed. After digestion of the hair with aqueous NaOH, the aimed the analytes were extracted with solid-phase extraction on diatomaceous earth columns, followed by derivatization with heptafluorobutyryl chloride and elution with n-hexane: propyl acetate (30:1, v/v) simultaneously. The method was linear (r 2 = 0.9982) in the concentration range of 0.5–200 pmol mL−1, reproducible (intra-day precision = 4.4–5.5 %, inter-day precision = 3.3–6.5 %), accurate (recoveries = 96.0–97.9 %) and sensitive (LOD = 0.1 pmol mL−1). The method was successfully applied for analyzing the hair tyramine from the control group, the patients with schizophrenia group and the drug abuse group. Compared with the control group, there is a significant difference to the patients with schizophrenia group, while there is no significant difference to the drug abuse group. Additionally, there is no significant difference between the drug abuse group and the patients with schizophrenia group. It is a clue that tyramine could be a biological marker for schizophrenia.