Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
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- 作者：Yanyan Li ; Hangzhou Wang ; Ting Sun ; Jinming Chen ; Lingchuan Guo…
- 关键词：Glioma stem cells ; Xenograft tumor ; A2B5 ; CD133
- 刊名：Molecular Cancer
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：14
- 期：1
- 全文大小：2,309 KB
- 参考文献：1. Yan, GN, Yang, L, Lv, YF, Shi, Y, Shen, LL, Yao, XH (2014) Endothelial cells promote stem-like phenotype of glioma cells through activating hedgehog pathway [J]. J Pathol 3: pp. 6
2. Wu, TF, Chen, JM, Chen, SS, Chen, GL, Wei, YX, Xie, XS (2013) Phenotype of SHG-44 glioma stem cell spheres and pathological characteristics of their xenograft tumors [J]. Zhonghua Zhong Liu Za Zhi 35: pp. 726-31
3. Gangoso, E, Thirant, C, Chneiweiss, H, Medina, JM, Tabernero, A (2014) A cell-penetrating peptide based on the interaction between c-Src and connexin43 reverses glioma stem cell phenotype [J]. Cell Death Dis 5: pp. e1023 CrossRef
4. Auf, G, Jabouille, A, Delugin, M, Guérit, S, Pineau, R, North, S (2013) High epiregulin expression in human U87 glioma cells relies on IRE1α and promotes autocrine growth through EGF receptor.[J]. BMC Cancer 13: pp. 597 CrossRef
5. Henderson, SD, Kimler, BF, Morantz, RA (1981) Radiation therapy of 9L rat brain tumors [J]. Int J Radiat Oncol Biol Phys 7: pp. 497-502 CrossRef
6. Ponten, J (1975) Neoplastic human glia cells in culture [M]. Human Tumor Cells in vitro [J]. Plenum Press, New York, pp. 175-85 CrossRef
7. Pavon, LF, Marti, LC, Sibov, TT, Malheiros, SM, Brandt, RA, Cavalheiro, S (2014) Gamarra. In vitro analysis of neurospheres derived from glioblastoma primary culture: a novel methodology paradigm [J]. Front Neurol 4: pp. 214 CrossRef
8. Fukaya, R, Ohta, S, Yamaguchi, M, Fujii, H, Kawakami, Y, Kawase, T (2010) Isolation of cancer stem-like cells from a side population of a human glioblastoma cell line, SK-MG-1 [J]. Cancer Lett 291: pp. 150-7 CrossRef
9. Dou, J, Gu, N (2010) Emerging strategies for the identification and targeting of cancer stem cells [J]. Tumour Biol 31: pp. 243-53 CrossRef
10. Gilbert, CA, Ross, AH (2009) Cancer stem cells: cell culture, markers, and targets for new therapies [J]. J Cell Biochem 108: pp. 1031-8 CrossRef
11. Clément, V, Marino, D, Cudalbu, C, Hamou, MF, Mlynarik, V, Tribolet, N (2010) Marker-independent identification of glioma-initiating cells [J]. Nat Methods 7: pp. 224-8 CrossRef
12. Lee, J, Kotliarova, S, Kotliarov, Y, Li, A, Su, Q, Donin, NM (2006) Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines [J]. Cancer Cell 9: pp. 391-403 CrossRef
13. Fael Al-Mayhani, TM, Ball, SL, Zhao, JW, Fawcett, J, Ichimura, K, Collins, PV (2009) An efficient method for derivation and propagation of glioblastoma cell lines that conserves the molecular profile of their original tumours [J]. J Neurosci Methods 176: pp. 192-9 CrossRef
14. Patru, C, Romao, L, Varlet, P, Coulombel, L, Raponi, E, Cadusseau, J (2010) CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors [J]. BMC Cancer 10: pp. 66 CrossRef
15. Pastrana, E, Silva-Vargas, V, Doetsch, F (2011) Eyes wide open: a critical review of sphere-formation as an assay for stem cells [J]. Cell Stem Cell 8: pp. 486-98 CrossRef
16. Hirschhaeuser, F, Menne, H, Dittfeld, C, West, J, Mueller-Klieser, W, Kunz-Schughart, LA (2010) Multicellular tumor spheroids: an underestimated tool is catching up again [J]. J Biotechnol 148: pp. 3-15
文摘
Objective The new glioma cell line SHG-139 was established and its phenotype, tumorigenicity, pathological characteristics, derived stem cells SHG139S were studied. Methods Immunohistochemistry was used to assess expressions in the patient and mouse tumor tissues, SHG-139 and SHG-139S. Primary SHG-139 culture was performed, cell proliferation, cell cycle and genetic characteristics were assessed. MiRNA (Micro RNA) and LncRNA (Long non-coding RNA) microarray was performed. Results We found that the glioma tissue was positive for A2B5 (Glial precursors ganglioside), GFAP (Glial fibrillary acidic protein), S-100 (Acid calcium bingding protein), VEGF (Vascular endothelial growth factor), VEGFR (Vascular endothelial growth factor receptor) and negative for Ki-67 (Nuclcar- associated antigen). SHG-139 proliferated significantly within 24h; its total number of chromosomes was 68; ratios of SHG-139 and SHG-139S cells in G1 phase were highest. SHG-139 cells were positive for A2B5, GalC (Galactocerebrosides), GFAP, S-100 and Vimentin, while SHG-139S cells were positive for A2B5, Nestin, and NG2 (Neuron-glia antigen2), and negative for Vimentin and IDHR132H (Isocitrate dehydrogenase); cells rarely stained for CD133 (Cluster of differentiation133). SHG-139 intracranial xenografts expressed GFAP, but no overt oligodendroglioma was observed. In SHG-139S xenografts, GFAP and S-100 were expressed, while CD133 was not detected; a few A2B5+ cells were found at tumor edges, and typical oligodendroglioma were obtained. In addition, SHG-139S xenograft tumors were more aggressive than those of SHG-139. Anti-mouse CD31 (Cluster of differentiation31) staining revealed murine vessels at the border between xenograft tumor and normal brain tissue; Anti-human CD34 (Cluster of differentiation34) staining was negative. Biochip technology of SHG139S showed several miRNA and lncRNA were differently expressed in SHG139 and SHG139S. Conclusions SHG-139 was an astroglioma cell line which yielded stem cells SHG-139S. SHG-139S cells constituted an A2B5+/CD133?/sup> GSC subgroup.