Purification and characterization of a new thermostable κ-carrageenase from the marine bacterium Pseudoalteromonas sp. QY203

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• 作者：Shangyong Li (1)
  Panpan Jia (1)
  Linna Wang (1)
  Wengong Yu (1)
  Feng Han (1)
  
• 关键词：κ ; carrageenase ; purification ; characterization ; thermostability ; Pseudoalteromonas
• 刊名：Journal of Ocean University of China
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：12
• 期：1
• 页码：155-159
• 全文大小：450KB
• 参考文献：1. Barbeyron, T., Gerard, A., Potin, P., Henrissat, B., and Kloareg, B., 1998. The kappa-carrageenase of the marine bacterium / Cytophaga drobachiensis. Structural and phylogenetic relationships within family-16 glycoside hydrolases. / Molecular Biology and Evolution, 15: 528-37. CrossRef
  2. Barbeyrona, T., Henrissatb, B., and Kloareg, B., 1994. The gene encoding the kappa-carrageenase of / Alteromonas carrageenovora is related to β-1,3-1,4-glucanases. / Gene, 139: 105-09. CrossRef
  3. Bernardo, R. Z., Yuridia, M. F., César, H. R., and Lourdes, V. T., 2004. Purification and characterization of lysine amino peptidase from / Kluyveromyces marxiamus. / FEMS Microbiology Letters, 235: 369-75.
  4. Collén, P. N., Lemoine, M., Daniellou, R., Guégan, J. P., Paoletti, S., and Helbert, W., 2009. Enzymatic degradation of κ-carrageenan in aqueous solution. / Biomacromolecules, 10: 1757-767. CrossRef
  5. Guibet, M., Colin, S., Barbeyron, T., Genicot, S., Kloareg, B., Michel, G., and Helbert, W., 2007. Degradation of lambda-carrageenan by / Pseudoalteromonas carrageenovora lambda-carrageenase: a new family of glycoside hydrolases unrelated to kappa- and iota-carrageenases. / Biochemical Journal, 404: 105-14. CrossRef
  6. Khambhaty, Y., Mody, K., and Jha, B., 2007. Purification and characterization of κ-carrageenase from a novel γ-proteobacterium, / Pseudomonas elongata (MTCC 5261) syn. / Microbulbifer elongatus comb. Nov. / Biotechnology and Bioprocess Engineering, 12: 668-75. CrossRef
  7. Kobayashi, T., Uchimura, K., Miyazaki, M., Nogi, Y., and Horikoshi, K., 2009. A new high-alkaline alginate lyase from a deep-sea bacterium / Agarivorans sp. / Extremophiles, 13: 121-29. CrossRef
  8. Laemmli, U. K., and Favre, M., 1973. Maturation of the head of bacteriophage T4. I. DNA packaging events. / Journal of Molecular Biology, 80: 575-99. CrossRef
  9. Liu, G. L., Li, Y., Chi, Z., and Chi, Z. M., 2011. Purification and characterization of κ-carrageenase from the marine bacterium / Pseudoalteromonas porphyrae for hydrolysis of κ-carrageenan. / Process Biochemistry, 46: 265-71. CrossRef
  10. Miller, G. L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. / Analytical Chemistry, 31: 426-28. CrossRef
  11. Michel, G., Barbeyron, T., Flament, D., Vernet, T., Kloareg, B., and Dideberg, O., 1999. Expression, purification, crystallization and preliminary x-ray analysis of the kappa-carrageenase from / Pseudoalteromonas carrageenovora. / Acta Crystallographica Section D-biological Crystallography, 55: 918-20. CrossRef
  12. Michel, G., Chantalat, L., Fanchon, E., Henrissat, B., Kloareg, B., and Dideberg, O., 2001. The iota-carrageenase of / Alteromonas fortis. A beta-helix fold-containing enzyme for the degradation of a highly polyanionic polysaccharide. / Journal of Biological Chemistry, 276: 40202-0209.
  13. Mou, H., Jiang, X. L., and Guan, H. S., 2003. A κ-carrageenan derived oligosaccharide prepared by enzymatic degradation containing anti-tumor activity. / Journal of Applied Phycology, 15: 297-03. CrossRef
  14. Potin, P., Sanseau, A., Le, G. Y., Rochas, C., and Kloareg, B., 1991. Purification and characterization of a new kappa-carrageenase from a marine / Cytophaga-like bacterium. / European Journal of Biochemistry, 201: 241-47. CrossRef
  15. Toshiyoshi, A., Yoshifumi, H., and Tatsuo, M., 1999. Purification and characterization of kappa-carrageenase from a marine bacterium / Vibrio sp. CA-1004. / Fisheries Science, 65: 937-42.
  16. Sun, F. X., Ma, Y. X., Wang, Y., and Liu, Q., 2010. Purification and characterization of novel κ-carrageenase from marine / Tamlana sp. HC4. / Chinese Journal of Oceanology and Limnology, 28: 1139-145. CrossRef
  17. Yuan, H., Song, J., Li, X., Li, N., and Dai, J., 2006. Immunomodulation and antitumor activity of κ-carrageenan oligosaccharides. / Cancer Letters, 243: 228-34. CrossRef
  18. Zhou, M. H., Ma, J. S., Li, J., Ye, H., Huang, K., and Zhao, X., 2008. A kappa-carrageenase from a newly isolated / Pseudoalteromonas-like bacterium, WZUC10. / Biotechnology and Bioprocess Engineering, 13: 545-51. CrossRef
  
• 作者单位：Shangyong Li (1)
  Panpan Jia (1)
  Linna Wang (1)
  Wengong Yu (1)
  Feng Han (1)
  
  1. Key Laboratory of Marine Drugs, Chinese Ministry of Education; Provincial Key Laboratory of Glycoscience and Glycotechnology of Shandong Province and School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, P. R. China
  
• ISSN：1993-5021

文摘

A new extracellular κ-carrageenase, namely CgkP, 34.0 kDa in molecular weight, was purified from Pseudoalteromonas sp. QY203. CgkP showed relatively high activity at acidities ranging from pH6.0 to pH9.0 and temperatures ranging from 30°C to 50°C with the highest activity at 45°C and pH7.2. Sodium chloride increased its activity markedly, and KCl increased its activity slightly. The divalent and trivalent metal ions including Cu2+, Ni2+, Zn2+, Mn2+, Al3+ and Fe3+ significantly inhibited its activity, while Mg2+ did not. CgkP remained 70% of original activity after being incubated at 40°C for 48 h, and remained 80% of the activity after being incubated at 45°C for 1 h. It exhibited endo-κ-carrageenase activity, mainly depolymerizing the κ-carrageenan into disaccharide and tetrasaccharide. CgkP was more thermostable than most of previously reported κ-carrageenases with a potential of being used in industry.