UPLC¨CMS Analysis for Simultaneous Determination of Eight Components in the ¡®Ge-Gen-Qin-Lian?Tablets

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• 作者：Rui An ; Ying Wang ; Lisha You and Xinhong Wang
• 关键词：Column liquid chromatography– ; mass spectrometry ; Ultra performance liquid chromatography ; Traditional Chinese Medicine ; ‘ ; Ge ; Gen ; Qin ; Lian’ ; tablets
• 刊名：Chromatographia
• 出版年：2009
• 出版时间：May, 2009
• 年：2009
• 卷：69
• 期：9-10
• 页码：969-975
• 全文大小：364.0 KB

文摘

A novel and rapid ultra performance liquid chromatography–mass spectrometry method was developed and validated for simultaneous determination of eight constituents in ‘Ge-Gen-Qin-Lian’ tablets. A UPLC BEH C₁₈ column (2.1 ¡Á 100 mm, 1.7 μm) was utilized in this method, and a mixture of methanol, 0.1 % formic acid, and 5 mmol L−1 ammonium acetate was adopted in the gradient elution program. The mass spectrometer equipped with an electrospray ionization source was performed in the selected-ion reaction (SIR) mode. A good linearity correlation (R 2 > 0.9991) was obtained over the investigated concentration ranges. Recoveries of this method for the eight components were from 96.00 to 100.6 % (RSD % < 3.4 % ).