Characterization of acetoin production in a budC gene disrupted mutant of Serratia marcescens G12

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• 作者：Songsong Gao (1)
  Wenyi Guo (1)
  Litao Shi (1)
  Yue Yu (1)
  Cuikun Zhang (1)
  Hongjiang Yang (1)
  
• 关键词：Acetoin ; Serratia marcescens ; budC mutant ; Expression
• 刊名：Journal of Industrial Microbiology and Biotechnology
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：41
• 期：8
• 页码：1267-1274
• 全文大小：682 KB
• 参考文献：1. Biswas R, Yamaoka M, Nakayama H, Kondo T, Yoshida K-I, Bisaria V, Kondo A (2012) Enhanced production of 2,3-butanediol by engineered / Bacillus subtilis. Appl Microbiol Biotechnol 94(3):651-58. doi:10.1007/s00253-011-3774-5 CrossRef
  2. Blomqvist K, Nikkola M, Lehtovaara P, Suihko ML, Airaksinen U, Straby KB, Knowles JK, Penttila ME (1993) Characterization of the genes of the 2,3-butanediol operons from / Klebsiella terrigena and / Enterobacter aerogenes. J Bacteriol 175(5):1392-404
  3. Celińska E, Grajek W (2009) Biotechnological production of 2,3-butanediol—current state and prospects. Biotechnol Adv 27(6):715-25. doi:10.1016/j.biotechadv.2009.05.002 CrossRef
  4. Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP (1998) A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked / Pseudomonas aeruginosa mutants. Gene 212(1):77-6 CrossRef
  5. Kovacikova G, Lin W, Skorupski K (2005) Dual regulation of genes involved in acetoin biosynthesis and motility/biofilm formation by the virulence activator AphA and the acetate-responsive LysR-type regulator AlsR in Vibrio cholerae. Mol Microbiol 57(2):420-33. doi:10.1111/j.1365-2958.2005.04700.x CrossRef
  6. Liu Z, Qin J, Gao C, Hua D, Ma C, Li L, Wang Y, Xu P (2011) Production of (2S,3S)-2,3-butanediol and (3S)-acetoin from glucose using resting cells of / Klebsiella pneumonia and / Bacillus subtilis. Bioresour Technol 102(22):10741-0744. doi:10.1016/j.biortech.2011.08.110 CrossRef
  7. Lopez J, Thoms B, Fortnagel P (1973) Mutants of / Bacillus subtilis blocked in acetoin reductase. Eur J Biochem 40(2):479-83 CrossRef
  8. Moons P, Van Houdt R, Vivijs B, Michiels CW, Aertsen A (2011) Integrated regulation of acetoin fermentation by quorum sensing and pH in / Serratia plymuthica RVH1. Appl Environ Microbiol 77(10):3422-427. doi:10.1128/AEM.02763-10 CrossRef
  9. Nakashimada Y, Marwoto B, Kashiwamura T, Kakizono T, Nishio N (2000) Enhanced 2,3-butanediol production by addition of acetic acid in Paenibacillus polymyxa. J Biosci Bioeng 90(6):661-64
  10. Nicholson WL (2008) The / Bacillus subtilis ydjL (bdhA) gene encodes acetoin reductase/2,3-butanediol dehydrogenase. Appl Environ Microbiol 74(22):6832-838. doi:10.1128/AEM.00881-08 CrossRef
  11. Qin H, Yang H, Qiao Z, Gao S, Liu Z (2013) Identification and characterization of a / Bacillus subtilis strain HB-1 isolated from Yandou, a fermented soybean food in China. Food Control 31(1):22-7. doi:10.1016/j.foodcont.2012.10.004 CrossRef
  12. Rao B, Zhang L, Sun Ja.Su G, Wei D, Chu J, Zhu J, Shen Y (2012) Characterization and regulation of the 2,3-butanediol pathway in / Serratia marcescens. Appl Microbiol Biotechnol 93(5):2147-159. doi:10.1007/s00253-011-3608-5 CrossRef
  13. Reese MG (2001) Application of a time-delay neural network to promoter annotation in the Drosophila melanogaster genome. Comput Chem 26(1):51-6 CrossRef
  14. Schweizer HP (1991) Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97(1):109-21 CrossRef
  15. Shi L, Gao S, Yu Y, Yang H (2014) Microbial production of 2,3-butanediol by a newly-isolated strain of / Serratia marcescens. Biotechnol Lett 36(5):969-73. doi:10.1007/s10529-013-1433-x CrossRef
  16. Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li W, Lopez R, McWilliam H, Remmert M, Soding J, Thompson JD, Higgins DG (2011) Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol 7:539. doi:10.1038/msb.2011.75 CrossRef
  17. Sun J-A, Zhang L-Y, Rao B, Shen Y-L, Wei D-Z (2012) Enhanced acetoin production by / Serratia marcescens H32 with expression of a water-forming NADH oxidase. Bioresour Technol 119:94-8. doi:10.1016/j.biortech.2012.05.108 CrossRef
  18. Sun J, Zhang L, Rao B, Han Y, Chu J, Zhu J, Shen Y, Wei D (2012) Enhanced acetoin production by / Serratia marcescens H32 using statistical optimization and a two-stage agitation speed control strategy. Biotechnol Bioproc Eng 17(3):598-05. doi:10.1007/s12257-011-0587-4 CrossRef
  19. Totten PA, Lory S (1990) Characterization of the type a flagellin gene from / Pseudomonas aeruginosa PAK. J Bacteriol 172(12):7188-199
  20. Van Houdt R, Aertsen A, Michiels CW (2007) Quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in / Aeromonas hydrophila AH-1?N. Res Microbiol 158(4):379-85. doi:10.1016/j.resmic.2006.11.015 CrossRef
  21. Van Houdt R, Moons P, Hueso Buj M, Michiels CW (2006) N-acyl-L-homoserine lactone quorum sensing controls butanediol fermentation in Serratia plymuthica RVH1 and / Serratia marcescens MG1. J Bacteriol 188(12):4570-572. doi:10.1128/JB.00144-06 CrossRef
  22. Yang H, Matewish M, Loubens I, Storey DG, Lam JS, Jin S (2000) migA, a quorum-responsive gene of / Pseudomonas aeruginosa, is highly expressed in the cystic fibrosis lung environment and modifies low-molecular-mass lipopolysaccharide. Microbiology 146(Pt 10):2509-519
  23. Zhang X, Zhang R, Yang T, Zhang J, Xu M, Li H, Xu Z, Rao Z (2013) Mutation breeding of acetoin high producing / Bacillus subtilis blocked in 2,3-butanediol dehydrogenase. World J Microbiol Biotechnol 29(10):1783-789. doi:10.1007/s11274-013-1339-8 CrossRef
  24. Zhang Y, Li S, Liu L, Wu J (2013) Acetoin production enhanced by manipulating carbon flux in a newly isolated / Bacillus amyloliquefaciens. Bioresour Technol 130:256-60. doi:10.1016/j.biortech.2012.10.036 CrossRef
  
• 作者单位：Songsong Gao (1)
  Wenyi Guo (1)
  Litao Shi (1)
  Yue Yu (1)
  Cuikun Zhang (1)
  Hongjiang Yang (1)
  
  1. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China
  
• ISSN：1476-5535

文摘

The 2,3-butanediol (2,3-BD) dehydrogenase gene budC of Serratia marcescens G12 was disrupted to construct the acetoin (AC) producing strain G12M. In shake-flask cultures, AC production was enhanced by increased concentrations of glucose or sodium acetate in G12M. In fed-batch fermentation, G12M produced 47.5?g/L AC along with 9.8?g/L 2,3-BD. The expression of the key enzymes for AC synthesis was further investigated. Alpha-acetolactate synthase gene budB decreased its expression significantly in G12M compared with G12. This probably explained the moderate AC production in G12M cultures. Additionally, overexpression of budB gene and α-acetolactate decarboxylase gene budA was conducted in G12M and no significant increase of AC was observed. The results suggested that intracellular AC accumulation might inhibit the expression of budB and budA gene and induce budC gene expression in G12M. Our analyses offered the bases for further genetic manipulations in improving AC production in microbial fermentations.