Staurosporine from the endophytic Streptomyces sp. strain CNS-42 acts as a potential biocontrol agent and growth elicitor in cucumber
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- 作者：Xiaolin Li (1) (3)
Pei Huang (2) (4)
Qian Wang (2) (4)
Lie Xiao (1)
Miaomiao Liu (2) (4)
Krishna Bolla (2)
Bo Zhang (1)
Linyong Zheng (3)
Bingcheng Gan (3)
Xueting Liu (2)
Lixin Zhang (2)
Xiaoping Zhang (1) - 关键词：Chinese medicinal plants ; Endophytic actinobacteria ; Antimicrobial activity ; Plant growth promoter ; Biocontrol ; Cucumber ; Staurosporine
- 刊名：Antonie van Leeuwenhoek
- 出版年：2014
- 出版时间：September 2014
- 年：2014
- 卷：106
- 期：3
- 页码：515-525
- 全文大小：4,018 KB
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Pei Huang (2) (4)
Qian Wang (2) (4)
Lie Xiao (1)
Miaomiao Liu (2) (4)
Krishna Bolla (2)
Bo Zhang (1)
Linyong Zheng (3)
Bingcheng Gan (3)
Xueting Liu (2)
Lixin Zhang (2)
Xiaoping Zhang (1)
1. Department of Microbiology, College of Resource and Environment, Sichuan Agricultural University, Chengdu, 611130, China
3. Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China
2. Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
4. University of Chinese Academy of Sciences, Beijing, 100049, China - ISSN：1572-9699
文摘
Chinese medicinal plants and their surrounding rhizospheric soil serve as promising sources of actinobacteria. A total of 180 actinobacteria strains were isolated from the rhizosphere soil, leaves, stems, and roots of nine selected plants and have been identified as potential biocontrol agents against Fusarium oxysporum f. sp. cucumerinum. An endophytic strain CNS-42 isolated from Alisma orientale showed the largest zone of inhibition demonstrating a potent effect against F. oxysporum f. sp. cucumerinum and a broad antimicrobial activity against bacteria, yeasts, and other pathogenic fungi. The in vivo biocontrol assays showed that the disease severity index was significantly reduced (P?P?Streptomyces sp. based on cultural, physiological, morphological characteristics, and 16S rRNA gene analysis. Further bioassay-guided isolation and purification revealed that staurosporine was responsible for its antifungal and plant growth promoting activities and the latter property of staurosporine is reported for the first time. The in vivo assay was further performed and indicated that staurosporine showed good growth promoting effect on the plant shoot biomass of cucumber. This is the first critical evidence identifying CNS-42 as a biocontrol agent for the soil borne pathogen, F. oxysporum f. sp. cucumerinum.