Identification of proteins associated with the production of methyl 5-(hydroxymethyl) furan-2-carboxylate toxin in Curvularia lunata

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• 作者：Jin-Xin Gao ; Tong Liu ; Jie Chen ; Ya-Qian Li
• 关键词：Cochliobolus lunatus ; DHFA ; Proteomics ; Toxin
• 刊名：Tropical Plant Pathology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：40
• 期：2
• 页码：119-126
• 全文大小：
• 参考文献：Chen J, Yan HH, Gao ZG, Xue CS, Zhuang JH (2003) Identification techniques for physiological differentiation of Curvularia lunata in maize. Acta Phytopathol Sin 33:121鈥?25
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• 作者单位：Jin-Xin Gao (1) (2)
  Tong Liu (1) (2) (3)
  Jie Chen (1) (2)
  Ya-Qian Li (1) (2)
  
  1. School of Agriculture and Biology, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, China
  2. State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai, 200240, China
  3. School of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, 163319, Heilongjiang, China
  
• 刊物类别：Plant Pathology;
• 刊物主题：Plant Pathology;
• 出版者：Springer International Publishing
• ISSN：1983-2052

文摘

Curvularia lunata produces a non-host specific toxin known as methyl 5-(hydroxymethyl) furan-2-carboxylate (M5HF2C), a determinant of high virulence to maize. We constructed mutants through Agrobacterium tumefaciens-mediated transformation and found that mutant T806 showed a significant decline in pathogenicity on maize leaves and produced no detectable M5HF2C. Although the tagged gene (Clt-1) has been cloned and demonstrated to be closely association with M5HF2C production and pathogenicity, we expected hypothesized that other proteins might also be related to toxin production. Therefore we used two-dimensional gel electrophoresis to profile T806, and found that there were two up-regulated and six missing proteins in T806 compared to C. lunata wild-type CX-3. These results indicated that these proteins were associated with signal transduction, protein synthesis, secondary metabolism and pathogenicity. Real-time quantitative PCR analysis of five selected genes showed a correlation between low gene expression and detection of the corresponding proteins. Of these, dihydropteroate (DHFA) synthase may be more likely involved in M5HF2C biosynthesis. Bioinformatics analysis showed that DHFA synthase consists of 469 amino acids, which is clustered into one branch with dothideomycete DHFA synthase homolologs in a phylogenetic tree. Further study on the exact role of the DHFA synthase gene is underway. Keywords Cochliobolus lunatus DHFA Proteomics Toxin