Octacosanol Enhances the Proliferation and Migration of Human Umbilical Vein Endothelial Cells via Activation of the PI3K/Akt and MAPK/Erk Pathways

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• 作者：Yu-Wei Liu ; Pei-Yuan Zuo ; Xiang-Nan Zha ; Xing-Lin Chen ; Rong Zhang ; Xiao-Xiao He…
• 关键词：Octacosanol ; Migration ; Proliferation ; Human umbilical vein endothelial cells ; PI3K/Akt signaling pathway ; MAPK/Erk1/2 signaling pathway ; p ; Akt ; p ; Erk1/2
• 刊名：Lipids
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：50
• 期：3
• 页码：241-251
• 全文大小：3,677 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Life Sciences
  Biochemistry
  Medicinal Chemistry
  Microbial Genetics and Genomics
  Nutrition
  Bioorganic Chemistry
  Medical Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9307

文摘

Atherosclerosis is characterized by endothelial dysfunction, lipid deposition, fibro-proliferative reactions and inflammation. Octacosanol is a high-molecular-weight primary aliphatic alcohol. As the main component of a cholesterol-lowering drug, octacosanol could inhibit lipids accumulation and cholesterol metabolism. To explore the indication of octacosanol on endothelial protection, we evaluated its effects on the proliferation and migration of human umbilical vein endothelial cells (HUVEC). Cell viability assay using methyl thiazolyl tetrazolium and 5-ethynyl-2-deoxyuridine revealed that 3.125?μg/ml octacosanol promoted the proliferation of HUVEC. A cell migration assay indicated that 0.781 and 3.125?μg/ml octacosanol increased the migration of HUVEC. Moreover, the phosphorylation levels of Akt and Erk1/2 were significantly elevated under exposure to octacosanol. Blocking the activation of Akt and Erk with their potent inhibitors LY294002 and PD98059, respectively, markedly attenuated the octacosanol-induced proliferation and migration of HUVEC. These findings demonstrated for the first time that octacosanol enhanced the proliferation and migration of HUVEC and mediated these effects through activation of the PI3K/Akt and MAPK/Erk1/2 signaling pathways.