Expression of dmrt1 and sox9 during gonadal development in the Siberian sturgeon (Acipenser baerii)

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• 作者：Julio Berbejillo (1)
  Anabel Martinez-Bengochea (1)
  Gabriela Bedó (2)
  Denise Vizziano-Cantonnet (1)
  
• 关键词：Sturgeons ; Sex differentiation ; Gonad morphology ; Gene expression
• 刊名：Fish Physiology and Biochemistry
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：39
• 期：1
• 页码：91-94
• 全文大小：210KB
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• 作者单位：Julio Berbejillo (1)
  Anabel Martinez-Bengochea (1)
  Gabriela Bedó (2)
  Denise Vizziano-Cantonnet (1)
  
  1. Laboratorio de Fisiología de la Reproducción y Ecología de Peces, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Iguá 4225, 11400, Montevideo, Uruguay
  2. Sección Genética Evolutiva, Instituto de Biología, Facultad de Ciencias, Universidad de la República Oriental del Uruguay, Montevideo, Uruguay
  
• ISSN：1573-5168

文摘

The sex differentiation period of the Siberian sturgeon was investigated through expression profiling of two testicular markers (dmrt1 and sox9). At the molecular level, a clear sexual dimorphism of dmrt1 and sox9 was observed in 3-year-old fish with immature gonads, in which males showed higher expression of these genes. Among 16-month-old sturgeons cultured in Uruguay, gonad morphology analyses showed one group of fish with undifferentiated gonads and a second group which had started their histological differentiation into ovaries or testes. dmrt1 showed a significantly higher expression in testes of recently differentiated fish, but this was not the case for sox9. In undifferentiated fish, we observed two clearly different groups in terms of expression: one group of fish over-expressing male markers (dmrt1, sox9) and another group of fish showing very low expression of these genes. This suggests that fish undergoing male differentiation can be identified by their profiles of gene expression before they undergo morphological differentiation.