Developmental Upregulation of an Alternative Form of pcp2 with Reduced GDI Activity

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• 作者：Jaroslaw J. Barski (1) (2)
  Brad M. Denker (3)
  Jiazhen Guan (3)
  Matthias Lauth (4)
  Fabio Spreafico (5)
  Andrzej Fertala (6)
  Michael Meyer (7)
  
• 关键词：pcp2 ; L7 ; Splice variant ; Cerebellum
• 刊名：The Cerebellum
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：13
• 期：2
• 页码：207-214
• 全文大小：660 KB
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• 作者单位：Jaroslaw J. Barski (1) (2)
  Brad M. Denker (3)
  Jiazhen Guan (3)
  Matthias Lauth (4)
  Fabio Spreafico (5)
  Andrzej Fertala (6)
  Michael Meyer (7)
  
  1. Center for Experimental Medicine, Medical University of Silesia, ul. Medyk?w 4, 40-752, Katowice, Poland
  2. Department of Physiology, Medical University of Silesia, 40-752, Katowice, Poland
  3. Department of Medicine, Renal Division, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, 02115, USA
  4. Institute of Molecular Biology and Tumor Research, Philipps Universit?t, 35032, Marburg, Germany
  5. Formerly Max-Planck-Institute of Neurobiology, 82152, Martinsried, Germany
  6. Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA, 19107, USA
  7. Institute of Physiology, Ludwig-Maximilians University, 80336, Munich, Germany
  
• ISSN：1473-4230

文摘

The pcp2/L7 gene is characterized by its very cell type-specific expression restricted to cerebellar Purkinje cells and retinal bipolar neurons. Although remarkable progress as to the biochemical properties of the encoded protein has been made, knowledge on its physiological functions remains sparse. While characterizing a pcp2-driven transgenic strain, we observed the presence of a longer, so far unknown, pcp2 transcript. Different from another recently discovered splice variant, ret-pcp2, expression of this novel transcript is observed in bipolar as well as cerebellar Purkinje cells of mid-postnatal mice. The protein encoded by our novel variant appears to be less efficient in binding to Gα subunits compared to the original L7/pcp2 protein and it is also less inhibitory with respect to GTPγ binding. Its expression in the eye appears to be independent from eye opening in postnatal mice.