Low temperature caused modifications in the arrangement of cell wall pectins due to changes of osmotic potential of cells of maize leaves (Zea mays L.)
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- 作者：Anna Bilska-Kos ; Danuta Solecka ; Aleksandra Dziewulska ; Piotr Ochodzki…
- 关键词：Cell wall ; Cold stress ; Osmotic potential ; Pectins ; Zea mays
- 刊名：Protoplasma
- 出版年：2017
- 出版时间：March 2017
- 年：2017
- 卷：254
- 期：2
- 页码：713-724
- 全文大小：2259KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Cell Biology; Plant Sciences; Zoology;
- 出版者：Springer Vienna
- ISSN：1615-6102
- 卷排序：254
文摘
The cell wall emerged as one of the important structures in plant stress responses. To investigate the effect of cold on the cell wall properties, the content and localization of pectins and pectin methylesterase (PME) activity, were studied in two maize inbred lines characterized by different sensitivity to cold. Low temperature (14/12 °C) caused a reduction of pectin content and PME activity in leaves of chilling-sensitive maize line, especially after prolonged treatment (28 h and 7 days). Furthermore, immunocytohistological studies, using JIM5 and JIM7 antibodies, revealed a decrease of labeling of both low- and high-methylesterified pectins in this maize line. The osmotic potential, quantified by means of incipient plasmolysis was lower in several types of cells of chilling-sensitive maize line which was correlated with the accumulation of sucrose. These studies present new finding on the effect of cold stress on the cell wall properties in conjunction with changes in the osmotic potential of maize leaf cells.