Glial-Like Differentiation Potential of Human Mature Adipocytes

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• 作者：Antonella Poloni (1)
  Giulia Maurizi (1)
  Federica Foia (1)
  Eleonora Mondini (2)
  Domenico Mattiucci (1)
  Patrizia Ambrogini (3)
  Davide Lattanzi (3)
  Stefania Mancini (1)
  Massimo Falconi (4)
  Saverio Cinti (2)
  Attilio Olivieri (1)
  Pietro Leoni (1)
  
  1. Clinica di Ematologia ; Dipartimento di Scienze Cliniche e Molecolari ; Universit脿 Politecnica delle Marche ; Via Tronto ; 60020 ; Ancona ; Italy
  2. Dipartimento di Medicina Sperimentale e Clinica ; Universit脿 Politecnica delle Marche ; Ancona ; Italy
  3. Dipartimento di Scienze della Terra ; della Vita e dell鈥橝mbiente ; Sezione di Fisiologia ; Universit脿 di Urbino Carlo Bo ; Urbino ; Italy
  4. Clinica Chirurgia del Pancreas ; Universit脿 Politecnica delle Marche ; Ospedali Riuniti ; Ancona ; Italy
  
• 关键词：Dedifferentiated adipocytes ; Neurosphere ; Differentiation ; Glial ; like cells
• 刊名：Journal of Molecular Neuroscience
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：55
• 期：1
• 页码：91-98
• 全文大小：622 KB
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• 刊物主题：Neurosciences; Neurochemistry; Cell Biology; Proteomics; Neurology;
• 出版者：Springer US
• ISSN：1559-1166

文摘

The potential ability to differentiate dedifferentiated adipocytes into a neural lineage is attracting strong interest as an emerging method of producing model cells for the treatment of a variety of neurological diseases. Here, we describe the efficient conversion of dedifferentiated adipocytes into a neural-like cell population. These cells grew in neurosphere-like structures and expressed a high level of the early neuroectodermal marker Nestin. These neurospheres could proliferate and express stemness genes, suggesting that these cells could be committed to the neural lineage. After neural induction, NeuroD1, Sox1, Double Cortin, and Eno2 were not expressed. Patch clamp data did not reveal different electrophysiological properties, indicating the inability of these cells to differentiate into mature neurons. In contrast, the differentiated cells expressed a high level of CLDN11, as demonstrated using molecular method, and stained positively for the glial cell markers CLDN11 and GFAP, as demonstrated using immunocytochemistry. These data were confirmed by quantitative results for glial cell line-derived neurotrophic factor production, which showed a higher secretion level in neurospheres and the differentiated cells compared with the untreated cells. In conclusion, our data demonstrate morphological, molecular, and immunocytochemical evidence of initial neural differentiation of mature adipocytes, committing to a glial lineage.