Cytotoxic and apoptotic effects of menadione on rat hepatocellular carcinoma cells
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- 作者：Pinar Oztopcu-Vatan ; Muge Sayitoglu ; Melek Gunindi ; Emine Inan
- 关键词：Menadione ; HCC ; Therapeutic ; Cell morphology ; Cytotoxicity
- 刊名：Cytotechnology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：67
- 期：6
- 页码：1003-1009
- 全文大小：682 KB
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Muge Sayitoglu (2)
Melek Gunindi (3)
Emine Inan (3)
1. Department of Biology, Faculty of Arts and Sciences, Osmangazi University, Eskisehir, Turkey
2. Genetics Department, Institute for Experimental Medicine Research, Istanbul University, Istanbul, Turkey
3. Graduate School of Natural and Applied Sciences, Eskisehir Osmangazi University, Eskisehir, Turkey - 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Biotechnology
Biomedicine
Biochemistry - 出版者：Springer Netherlands
- ISSN：1573-0778
文摘
Hepatocellular carcinoma (HCC) is one of the most common cancers, which may lead to death. Menadione shows cytotoxic activity thought affecting redox cycling in cancer cells. The aim of the present study was to investigate the effects of menadione on rat hepatocellular carcinoma (H4IIE) cell morphology, cytotoxicity, apoptosis and DNA damage or repair in vitro. Cell morphology evaluated by microscopy and cell viability was determined using the 3-[4,5-dimethylthiazol-2yl]-diphenyltetrazolium bromide test. Apoptotic cell death was assessed in H4IIE cells treated with menadione by 4-6-diamidino-2-phenylindole staining. Quantitative real time polymerase chain reaction used to determine the expression level of poly (ADP-ribose) polymerase 1 (PARP1) gene. According to the results of this study menadione has got a cytotoxic activity (IC50 25 μM) and change the cell fate in H4IIE cells. Menadione treatments lead to PARP1 activation in a dose dependent manner and induce DNA damage and apoptosis, and this may suggest its use as a therapeutic agent in HCC treatment. Keywords Menadione HCC Therapeutic Cell morphology Cytotoxicity